水稻粳粳交花药培养继代愈伤组织分化培养基的优化

以继代的粳粳交（空育131／稻花香2号）F。花药培养的愈伤组织为试验材料，研究培养基中的影响因素，对已有的培养基进行优化。共用20种培养基进行分化，涉及7种影响因素：谷氨酰胺，糖类，激素，山梨醇，铜、银离子组合，硅素和活性炭。结果表明：（1）长时间继代的愈伤组织仍具有分化能力。（2）可以显著提高绿苗分化率的因素为60mg／L硅素，0.25mg／LAgNOs和0.75mg／L CuSO4·5H2O。（3）加160目活性炭的培养基中愈伤生长状况得到很大改善。（4）最佳分化培养基为G1W3。

Optimization of Differentiation Medium for Subcultured Calli Derived from Japonica/japonica Hybrid Rice Anther

The influencing factors of the medium were optimized for the differentiation of subcuhured anther calli, which were derived from japonica/japonica hybrid F1 （Kongyu 131/Daohuaxiang 2）. 20 culture mediums were devised for callus differentiation. Different combinations of 7 important factors were considered for the medium design. The 7 important factors included glutamine, carbohydrates, hormones, sorbitol, the composition of copper and silver ion, silicone, and activated charcoal. The results showed that： （1） The prolonged subcuhured calli continued to possess the differentiation ability. （2） The factor combination with 60 mg/L Silicon, 0.25 mg/L AgNO3 and 0.75mg/L CuSO45H2O were found to have the potential in significantly improving the differenti＇ation rate. （3） The callus growth conditions have been greatly improved through adding 160-mesh activated charcoal. （4） The best medium to differentiate subcultured calli was G1W3.