Studies on the immunolabeling of heartwood extractives: characterization of anti-agatharesinol antiserum |
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Authors: | Takeshi?Nagasaki Norihisa?Osada Mika?Sato Seiichi?Yasuda Koetsu?Takahashi Email author" target="_blank">Takanori?ImaiEmail author |
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Institution: | (1) Laboratory of Biomass Resources Utilization, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan;(2) Laboratory of Forest Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan;(3) Section of Forest Resources, Department of Bioenvironment, Faculty of Agriculture, Yamagata University, Tsuruoka, 997-8555, Japan |
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Abstract: | The reactivity of previously prepared antiserum against agatharesinol, a norlignan, was determined by competitive inhibition–ELISA (enzyme-linked immunosorbent assay) using several natural compounds that are representative of plant extractives as competitive inhibitors. The antiserum strongly reacted with some norlignans (i.e., agatharesinol, hinokiresinol, and metasequirin C), each of which has a common chemical-structural unit. This result suggests that the antiserum recognizes the specific chemical-structural unit composing those norlignans. Moreover, differences in the reactivities of the antiserum with agatharesinol derivatives and synthetic chalcones propose the trans-3-p-hydroxyphenyl-1-phenylpropene structural unit as the major antigenic determinant for the antiserum.Part of this paper was presented at the 45th Lignin Symposium, Matsuyama, October 2000 and the 51th Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001 |
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Keywords: | Antibody Agatharesinol Norlignan Competitive inhibition– ELISA |
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