拮抗菌X23发酵液对金黄色葡萄球菌的抑制作用

为了解拮抗菌X23发酵液对金黄色葡萄球菌的杀菌作用及机理,将拮抗菌X23发酵液与金黄色葡萄球菌作用后,运用琼脂平板倾注法检测菌体克隆数,扫描电镜观察菌体形态,透射电镜观察菌体超微结构。结果表明:拮抗菌X23发酵液与金黄色葡萄球菌作用60、90、120 min后,金黄色葡萄球菌克隆数极显著低于生理盐水对照组(P<0.01);与拮抗菌X23发酵液作用60 min后,金黄色葡萄球菌菌体表面出现囊状小突起,细胞膜皱褶并最终破裂,菌体内出现大块透电子区,胞浆内容物稀疏,胞质不均匀,胞内空泡变性加重,分裂相减少,菌体溶解。推测拮抗菌X23发酵液可以通过破坏细胞膜结构而杀死金黄色葡萄球菌。

The bactericidal effect of antagonistic bacterium X23 fermentation liquid against Staphylococcus aureus

Abstract: Staphylococcus aureus was treated with antagonistic bacterium X23 fermentation liquid, then the number of bacterial colonies was counted using agar plate pouring method, and the morphology and ultrastructure of the bacteria were observed using scanning electron-microscope (SEM) and transmission electron- microscope (TEM) respectively to study the bactericidal effect and partial mechanism of antagonistic bacterium X23 fermentation liquid on S. aureus. The result showed that the number of S. aureus colonies in the treated groups was significantly lower than that in the control group (P<0.01). In the group treated for 60 min, the surface of S. aureus had many small protuberances and the cell membrane was folded and eventually ruptured, inside the bacterial cell, massive electron penetration areas appeared, contents in cytoplasm became sparse and uneven, intracellular vacuolar degeneration was aggravated. Besides, cell division was reduced, and bacterium cells were dissolved. These results indicated that antagonistic bacterium X23 fermentation liquid could kill S. aureus through disrupting the bacterial structure.