| 水稻抗白叶枯病基因Xa21转基因水稻及其杂交稻研究 |
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| 引用本文： | 吴家道,杨剑波,许传万,李莉,向太和,倪大虎,汪秀峰,贾士荣,唐益雄,张世平. 水稻抗白叶枯病基因Xa21转基因水稻及其杂交稻研究[J]. 作物学报, 2001, 27(1): 29-34 |
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| 作者姓名： | 吴家道 杨剑波 许传万 李莉 向太和 倪大虎 汪秀峰 贾士荣 唐益雄 张世平 |
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| 作者单位： | 安徽省农业科学院水稻研究所， 农业部水稻遗传育种重点开发实验室， 安徽合肥 230031 |
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| 基金项目： | 本项研究前期工作得到美国RogorN Beachy博士的帮助， 他现在工作单位是Donald Danforth Plant Science Center(St. Louis, Mo.)， 同时也得到洛克菲勒基金(Rockefeller Foundation)的资助，在此一并致谢。 |
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| 摘 要： | 用基因枪转基因技术将高抗白叶枯病的Xa21基因导入中国三系杂交稻恢复系(明恢63)和保持系(皖B)中，获得转基因水稻系，其中4个株系只含有Xa21基因，不含选择标记潮霉素抗性基因hph。筛选抗白叶枯病转基因纯合系，在田间种植6代，用PCR检测证明，Xa21基因能稳定遗传表达。用转基因水稻配制两系杂交稻，杂交组合F1含有Xa21基因
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| 关 键 词： | Xa21基因 转基因水稻 杂交稻 |
| 收稿时间： | 2000-02-22 |
| 修稿时间： | 2000-02-22 |
Study on Resistance Geneto Bacterial Blight Xa21 Transgenic Rice and Their Hybrid Combinations |
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| WU Jia-Dao,YANG Jian-Bo,XU Chuan-Wan,LI Li,XIANG Tai-He,NI Da-Fu,WANG Xiu-Feng,JIA Shi-Rong,TANG Yi-Xiong,ZHANG Shi-Ping,Claude M. Fauquet. Study on Resistance Geneto Bacterial Blight Xa21 Transgenic Rice and Their Hybrid Combinations[J]. Acta Agronomica Sinica, 2001, 27(1): 29-34 |
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| Authors: | WU Jia-Dao YANG Jian-Bo XU Chuan-Wan LI Li XIANG Tai-He NI Da-Fu WANG Xiu-Feng JIA Shi-Rong TANG Yi-Xiong ZHANG Shi-Ping Claude M. Fauquet |
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| Abstract: | Xa21 gene and h ph gene were co-transferred into Chinese 3-line hybrid rice lines Minghui 63(restorer line) and Wan B (maintainence line )by biolistics gene transfer method. A total of 5 transgenic rice lines with enhanced resistance against rice bacterial blight (BB) were obtained. Four of the transgenic lines were found to have only Xa21 gene integrated into the genome, and the other one have both Xa21 and hph integrated. Molecular analysis to the transgenic plants (up to six generations) showed that Xa21 gene had been integrated into the plant genome. The offsprings inherited the resistance from Xa21 stable. The Xa21 transgenic rice plants have been used as the restorer line for 2-line hybrid combinations. F1 of some combinations were found to show strong BB resistance and high yield-increasing potentiality. |
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| Keywords: | Xa21 gene Transgenic rice Hybrid rice |
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