Pb~(2+)·Hg~(2+)对平菇菌丝生长的影响及中微量元素对Hg~(2+)的拮抗解毒效应

目的]研究Pb^2＋、Hg^2＋对平菇菌丝生长的影响，探索元素Ca^2＋、Mg^2＋、Fe^2＋与Hg^2＋之间的拮抗效应。方法]以平菇1012菌株为材料。采用舍不同浓度梯度重金属及中微量元素的培养基进行平板培养试验，观察、测定菌丝生长情况。结果]Pb^2＋浓度为0．5—8．0mg／L对平菇菌丝的生长没有不良影响，甚至有不显著的促进作用，Pb^2＋浓度高于10．0mg／L，抑制平菇菌丝的生长，Pb^2＋浓度达到50．0mg／L时，平菇菌丝的生长受到极显著抑制（P≤0．003），当Pb^2＋浓度≥100．0mg／L时，菌丝萌发后立即陷入停顿、死亡。Hg^2＋对平菇的毒性强于Pb^2＋，从其低浓度开始就对平菇菌丝的生长表现抑制作用，当Hg^2＋浓度为15mg／L时，抑制作用显著（P〈0．05）。向含浓度15mg／LHg^2＋的平板培养基中分别添加碱土元素Ca^2＋、Mg^2＋和微量元素Fe^2＋，对Hg^2＋抑制平菇菌丝生长的毒害有一定的拮抗解毒效果．英中又以添加浓度1000mg／L的Ca^2＋效果最好。结论]该研究对于控制食用菌重金属富集有重要的参考价值。

Study on the Effects of Pb~(2+),Hg~(2+) on Mycelium Growth of Pleurotus ostreatus and Antagonistic Effects of Alkaline-earth and Trace Elements

Objective]The research aimed to study the effects of Pb2+ and Hg2+ on mycelium growth of Pleurotus ostreatus and the antagonistic effects of them with alkaline-earth and trace elements.Method]Taken P.ostreatus 1012 for materials and cultured with different culture media contained different concentrations heavy metals,alkaline-earth and trace elements.Then the growth situations were observed and determined.Result]Pb2+ showed no inhibition or even insignificant improvement effects on the mycelium growth of...