白喉型鸡痘病毒Gibbs株免疫特性研究

白喉型鸡痘病毒Gibbs株能在鸡胚绒毛尿囊膜上良好增殖，接种后１２０小时，绒毛尿囊膜明显水肿，且淡黄色痘斑，其毒价达到１０^5.25-10^5.83EID50/0.2ml。用１０倍稀释的病毒悬液肌肉注射１３日龄雏鸡，无任何不良反应。用１００倍稀释病毒液皮下刺种免疫１４－４０日龄鸡，接种后３－６天，接种局部出现痘痂，接种后１４天，用１０倍稀释的Ｇibbs株病毒液二次皮下刺种或用鸡痘野毒株经腿部毛作擦，免疫未见任何局部反应或其它异常，保护率达１００％。，免疫后１６５天，保护率仍达８０％，试验结果证实该毒株具有良好的免疫原性和安全性。

ImmunologicalCharacterization of Diphterin Type Fowl Pox Virus Gibbs Strain

Gibbs strain,which is diphterin type fowl pox virus,may replicates well in 10-day-old embryonated chicken eggs via the dropped chorio allantoic membrane (CAM)route.120 hours post inoculation(pi),the swelling and pox reactions was observed in CAM.Moreover,the titer of virus suspersion is 105.25～105.83EID50 per 0.2ml.The minimal immune dose by the conventional wing-web route was 10-5 0.1ml.13-day-old chickens,inoculated by intramuscular injection with 1∶10 virus dilution,no abnormal signs was observed.Vaccinated chickens with 1∶100 dilution,developing lesion at site of inoculation.At 14 day post inoculation,these immuned chickens were challenged with a virulent fowl pox strain HL or Gibbs strain,which protective rate was 100 percent,provided excellent protection.The duration of immunity lasted 5.5 months.