5种饵料对日本囊对虾早期生长及感染WSSV存活率的影响

采用人工配合饲料、鱼粉、丰年虫无节幼体、虾片和牡蛎肉5种不同饵料投喂日本囊对虾幼虾,实验周期20d,测量日本囊对虾的体长、体重及计算其成活率。结果表明,5种饵料对日本囊对虾生长的影响差异显著（P〈0.05）,其中丰年虫组体长、体重的增长明显优于其他各组,差异达极显著水平（P〈0.01）,牡蛎肉组次之,配合饲料组的体长、体重增长最小,鱼粉组体长、体重增长大于虾片组。人工投喂WSSV感染5种不同饵料投喂的日本囊对虾幼虾,实验周期10d。丰年虫组和鱼粉组的存活率最高,明显高于配合饲料和牡蛎肉两实验组,差异达极显著水平（P〈0.01）;丰年虫组和鱼粉组之间存活率无显著差异（P〉0.05）,人工配合饵料组和牡蛎肉组差异不显著（P〉0.05）。半定量PCR检测表明,感染前日本囊对虾幼虾均不携带WSSV,感染后全部个体均检测到相应的病毒特征片段。

Effects of Five Different Diets on Growth and WSSV Resistance of Marsupenaeus japonicus

Five diets,which were artificial feed,fish meal,artemia salina nauplii,shrimp flakes and oyster meat respectively,were used to feed M.japonicus,and the body length as well as body weight were measured,experimental period was 20 days. The results indicated that the effects of 5 species of diets on the growth of the M.japonicus were significantly different （P0.05）; the increasing of the weight and length of the group which fed with Artemia Salina nauplii were significantly higher than that of other groups （P0.01）,and the group which was fed with artificial feed got the slowest increasing in length and weight; the length and weight of the group which was fed with fish meal was higher than that of shrimp flakes; After infected with WSSV,and the experiment time was designed as 10 d,the survival rate of artemia salina nauplii group and the fish meal group were significantly higher than that of other groups （P0.01）; there was no significant difference between Artemia Salina nauplii group and the fish meal group（P0.05）,there was no significant difference between artificial feed group and the oyster meat group（P0.05）. The analysis results of semi-quantitive PCR showed that there were no WSSV gene fragments before infection but there were positive signs of infection after the experiment.