| 植物生长调节剂TDZ在草莓花药培养中的应用 |
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| 引用本文： | 王敬东,张丽,马洪爱,陈晓军,宋玉霞. 植物生长调节剂TDZ在草莓花药培养中的应用[J]. 农业科学与技术, 2010, 0(9): 82-84,90 |
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| 作者姓名： | 王敬东 张丽 马洪爱 陈晓军 宋玉霞 |
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| 作者单位： | 宁夏农林科学院农业生物技术研究中心,宁夏银川750002 |
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| 基金项目： | 宁夏回族自治区科技攻关项目（KGZ-16-07-02）;北京林业大学林禾育种国家工程实验室开放课题. |
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| 摘 要： | [目的]探讨植物生长调节剂TDE在草莓花药培养中的应用。[方法]采用不同浓度TDZ与NAA植物生长素进行组合,观察其对草莓愈伤组织诱导及其分化的影响。[结果]MS＋TDZ1.0mg/L＋NAA0.5mg/L适用于草莓愈伤组织的分化,分化率高达75%,但TDZ对草莓愈伤组织的诱导无显著作用。[结论]该研究结果为草莓通过花药培养途径进行规模生产提供参考。
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| 关 键 词： | 草莓花药 TDZ 愈伤组织 不定芽 |
Application of Plant Growth Regulator TDZ in Strawberry Anther Culture |
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| WANG Jing-dong,ZHANG Li,MA Hong-ai,CHEN Xiao-jun,SONG Yu-xia. Application of Plant Growth Regulator TDZ in Strawberry Anther Culture[J]. Agricultural Science ＆ Technology, 2010, 0(9): 82-84,90 |
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| Authors: | WANG Jing-dong ZHANG Li MA Hong-ai CHEN Xiao-jun SONG Yu-xia |
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| Affiliation: | Agricultural Biotechnology Center of Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002 |
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| Abstract: | [Objective] The aim was to investigate the application of plant growth regulator thidiazuron（TDZ）in strawberry anther culture.[Method]Different combinations of TDZ and naphthalene acetic acid（NAA）auxine with different concentrations were used for induction culture,and then its effect on callus induction and plant regeneration were observed.[Result] The combination of 1.0 mg/L TDZ＋0.5 mg/LNAA was effective to accelerating the differentiation and regeneration of strawberries,and the differentiation ratio was as high as 75%.However,TDZ showed insignificant effect on strawberries anther induction.[Conclusion]This research had provided reference for large-scale production of strawberry through anther culture. |
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| Keywords: | Strawberry anther TDZ Callus Adventitious bud |
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