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伏安极谱法测定槟榔提取物对过氧化氢自由基的清除活性
引用本文:黄玉林,祁静,唐敏,郑亚军,赵松林,陈良秋,陈卫军.伏安极谱法测定槟榔提取物对过氧化氢自由基的清除活性[J].热带作物学报,2010,31(2):314-318.
作者姓名:黄玉林  祁静  唐敏  郑亚军  赵松林  陈良秋  陈卫军
作者单位:中国热带农业科学院椰子研究所,海南省热带棕榈植物研究重点实验室(筹),海南文昌,571339
摘    要:采用微分脉冲伏安法及循环伏安法测定不同溶剂(水、氯仿、正已烷、丙酮、乙酸乙酯)槟榔提取物对过氧化氢(H2O2)自由基的清除能力。结果显示,用Vc和咖啡酸作对照,不同溶剂槟榔提取物均有清除过氧化氢自由基的能力,其中乙酸乙酯提取物的最高,水提取物的次之,氯仿、正已烷和丙酮提取物的清除能力略低。表明伏安极谱法可用作评价天然植物提取物清除H2O2活性大小的一种快速检测的分析方法。

关 键 词:槟榔  提取物  过氧化氢自由基  微分脉冲伏安法  循环伏安法

Determination of Hydrogen Peroxide Free Radical Scavenging Activity of Arecanut Extracts by Voltammetry Polarography Method
Huang Yulin,Qi Jing,Tang Min,Zheng Yajun,Zhao Songlin,Chen Liangqiu and Chen Wenjun.Determination of Hydrogen Peroxide Free Radical Scavenging Activity of Arecanut Extracts by Voltammetry Polarography Method[J].Chinese Journal of Tropical Crops,2010,31(2):314-318.
Authors:Huang Yulin  Qi Jing  Tang Min  Zheng Yajun  Zhao Songlin  Chen Liangqiu and Chen Wenjun
Institution:Huang Yulin,Qi Jing,Tang Min,Zheng Yajun,Zhao Songlin,Chen Liangqiu,Chen Wenjun Coconut Research Institute,CA TAS,Wenchang /Hainan Key Laboratory for Research of Tropical Palm Plants,Wenchang,Hainan 571339
Abstract:Areca nut extracts were prepared with different solvents(water,chloroform,n-hexane,acetone,ethyl acetate),and their hydrogen peroxide free radical scavenging activities were determined by using differential pulse polarography and cyclic voltammetry,with Vc and caffeic acid being used as control.The results showed that the areca nut extracts prepared with all the solvents had hydrogen peroxide free radical scavenging activities,in which the scavenging activity of the areaca nut extract prepared with ethyl ac...
Keywords:areca nut  extract  hydrogen peroxide free radical  differential pulse voltammetry polarography  cyclic voltammetry
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