| 用6个小麦─黑麦衍生系筛选与黑麦抗条锈病基因连锁的RAPD标记 |
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| 引用本文： | 陈建莉,薛秀庄,王祥正,许喜堂,RichardR-C.Wang.用6个小麦─黑麦衍生系筛选与黑麦抗条锈病基因连锁的RAPD标记[J].西北农业学报,1995,4(4):17-20. |
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| 作者姓名： | 陈建莉 薛秀庄 王祥正 许喜堂 RichardR-C.Wang |
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| 作者单位： | 陕西省小麦研究中心, 杨陵712100;陕西省小麦研究中心, 杨陵712100;陕西省小麦研究中心, 杨陵712100;陕西省小麦研究中心, 杨陵712100;USDA-ARS, FRRL, USU, Logan, UT84322-6300 |
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| 基金项目： | 国家自然科学基金;国家“八五”攻关和杨陵农业科技开发基金 |
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| 摘 要： | 用ＲＡＰＤ分析６个艺性状不同的小麦－麦抗条锈病衍生系材料，建立与黑麦抗条锈病基因连锁的分子标记。结果表明，所用６个随机引物中，４个引物在６个衍生系中扩增出６个黑麦特有的产物，其大小约１９７－９８０ｂｐ，分别被标记为Ｊ１７，Ｊ１８ａ，Ｊ１８ｂ，Ｊ１１ａ，Ｊ１１ｂ和Ｄ５。
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| 关 键 词： | 黑麦 条锈病 小麦 衍生系 抗病性 |
| 收稿时间： | 1994/10/28 0:00:00 |
| 修稿时间： | 1995/5/31 0:00:00 |
RAPD MARKERS FOR RYE CHROMATIN IN SIX WHEAT-RYE DERIVATIVES RESISTANT TO STRIPE RUST |
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| Chen Jianli,Xue Xiuzhuang,Wang Xiangzheng,Xu Xitang and Richard R-C. Wang.RAPD MARKERS FOR RYE CHROMATIN IN SIX WHEAT-RYE DERIVATIVES RESISTANT TO STRIPE RUST[J].Acta Agriculturae Boreali-occidentalis Sinica,1995,4(4):17-20. |
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| Authors: | Chen Jianli Xue Xiuzhuang Wang Xiangzheng Xu Xitang and Richard R-C Wang |
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| Institution: | Wheal Research Centre of Shaanxi Ptovince, Yangling 712100;Wheal Research Centre of Shaanxi Ptovince, Yangling 712100;Wheal Research Centre of Shaanxi Ptovince, Yangling 712100;Wheal Research Centre of Shaanxi Ptovince, Yangling 712100;USDA-ARS, FRRL, USU, Logan, UT84322-6300 |
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| Abstract: | Detection of rye gene for resistance to stripe rust using RAPD marker was performed using six wheat-rye derivatives.Four out of six primers yielded six specific products of rye (J17,J18 a,J18 b, J11 a,J11 b and D 5),of which J17,J11 b and D5 markers have been identified in all of six derivalives,J11 a in four derivatives, J18 a and J18 b only in three derivatives.RAPD assay for identifying rye or alien DNA fragment in wheat genetic background is suggested as a way to isolate new translocation in selection of early generation.Furthermore,in studying on alien gene,effect ef environment could be induced,breeding prcxedure and selection efficiency could be improved. |
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| Keywords: | Rye Stripe rust RAPD assay Molecular marker Wheat-rye derivatives Alien traslocation line Early generation selection |
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