| 甘蓝型油菜矮秆突变体的遗传及其矮秆基因的RAPD标记 |
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| 引用本文： | 黄天带,吴江生,王令强,华玉伟. 甘蓝型油菜矮秆突变体的遗传及其矮秆基因的RAPD标记[J]. 农业生物技术学报, 2006, 14(6): 942-945 |
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| 作者姓名： | 黄天带 吴江生 王令强 华玉伟 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,国家油菜改良武汉分中心,武汉,430070 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 甘蓝型油菜(BrassicanapusL.)矮秆突变体DS-1与中双四号配制正反交组合F1、F2、BC1和BC2,遗传分析表明,该突变体的矮秆性状受一对部分显性主效基因和微效基因控制,将这对显性主效基因命名为Ds1。同时随机选取147株F2植株作为Ds1的RAPD标记的筛选群体,最终从1041条随机引物中筛选到1条引物S470,其扩增的多态性片段S470.416与Ds1连锁,遗传距离为8.9cM。
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| 关 键 词： | 甘蓝型油菜 矮秆突变体 遗传分析 RAPD标记 |
| 文章编号： | 1006-1304（2006）06-0942-04 |
| 收稿时间： | 2006-01-10 |
| 修稿时间： | 2006-04-10 |
Genetic Analysis on a Dwarf Brassica napus Mutant and Screening RAPD Markers Linked to the Dwarf Gene |
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| HUANG Tian-dai,WU Jiang-sheng,WANG Ling-qiang,HUA Yu-wei. Genetic Analysis on a Dwarf Brassica napus Mutant and Screening RAPD Markers Linked to the Dwarf Gene[J]. Journal of Agricultural Biotechnology, 2006, 14(6): 942-945 |
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| Authors: | HUANG Tian-dai WU Jiang-sheng WANG Ling-qiang HUA Yu-wei |
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| Affiliation: | National Key Laboratory of Crop Genetic Improvement, National Rapeseed Improvement Center (Wuhan |
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| Abstract: | Genetic analysis of the dwarf stem were undertaken on crosses between a dwarf Brassica napus mutant DS-1 and a normal cultivar Zhongshuang 4. Inheritance of the dwarf stem in reciprocal F1, F2, BC1 and BC2 populations clearly revealed that the dwarf stem of DS-1 was controlled by a partial dominant major gene and some minor genes, and the partial major gene was named Ds1.At the same time the bulked-segregant method was used to screen RAPD markers linked to Ds1, of totally 1041 arbitrary primers screened for 147 randomly selected F2 plants, only the primer S470 produced repeatable marker S470.416 linked to Ds1, with a map distance of 8.9 cM. |
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| Keywords: | Brassica napus dwarf mutant genetic analysis RAPD markers |
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