| 鸡白痢沙门菌和鸡伤寒沙门菌PCR-RFLP鉴别 |
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| 引用本文： | 涂玉蓉,陈建红,任涛,张济培,司兴奎,牛森.鸡白痢沙门菌和鸡伤寒沙门菌PCR-RFLP鉴别[J].动物医学进展,2011,32(6):80-83. |
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| 作者姓名： | 涂玉蓉 陈建红 任涛 张济培 司兴奎 牛森 |
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| 作者单位： | 涂玉蓉,牛森,TU Yu-rong,NIU Sen(华南农业大学兽医学院,广东,广州,510642;佛山科学技术学院动物医学系,广东,佛山,528231);陈建红,张济培,司兴奎,CHEN Jian-hong,ZHANG Ji-pei,SI Xing-kui(佛山科学技术学院动物医学系,广东,佛山,528231);任涛,REN Tao(华南农业大学兽医学院,广东,广州,510642) |
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| 摘 要： | 根据鸡白痢沙门菌和鸡伤寒沙门菌flic基因可变区两端的保守序列设计1对引物,PCR扩增出约866 bp的产物,用HinplI对PCR产物进行酶切,经RFLP分析区分鸡白痢沙门菌和鸡伤寒沙门菌.利用该技术对1株鸡白痢沙门菌标准株及2株鸡伤寒沙门菌标准株进行分子鉴别,结果与预计的RFLP模式相符,证明该方法可行.在此基础上...
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| 关 键 词： | 鸡白痢沙门菌 鸡伤寒沙门菌 PCR-RFLP flie基因 鉴别 |
Differentiation of Salmonella pullorum from Salmonella gallinarum by PCR-RFLP |
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| TU Yu-rong,CHEN Jian-hong,REN Tao,ZHANG Ji-pei,SI Xing-kui,NIU Sen.Differentiation of Salmonella pullorum from Salmonella gallinarum by PCR-RFLP[J].Progress In Veterinary Medicine,2011,32(6):80-83. |
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| Authors: | TU Yu-rong CHEN Jian-hong REN Tao ZHANG Ji-pei SI Xing-kui NIU Sen |
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| Institution: | 1.2(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China;2.College of Life Science,Foshan University,Foshan,Guangdong,528231,China) |
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| Abstract: | According to the conserved sequence of the variable region of flic gene from S.pullorum and S.gallinarum,a pair of specific primers were designed.The PCR amplified product,866 bp fragment was digested with Hinp1I.S.pullorum and S.gallinarum were able to be differentiaed by PCR-RFLP.By using this method,the reference strains of S.pullorum and 2 reference strains of S.gallinarum were identified,with the results matching the predicted patterns.It could be concluded that this method is feasible.Furthermore,the isolates were differentiated,all of them were S.pullorum. |
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| Keywords: | PCR-RFLP |
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