恩诺沙星混悬液在猪体内残留消除规律研究

采用高效液相色谱法研究恩诺沙星(口服)混悬液在猪体内各组织中的残留消除规律.恩诺沙星(口服)混悬液,按每头猪10 mg/kg体重的剂量灌服给药,连续使用3 d之后,宰杀猪,取组织.组织样品经磷酸盐缓冲液提取,C18固相萃取柱净化,过膜,用流动相0.05 mol/L磷酸溶液/三乙胺一乙腈(82+18)溶解,微孔过滤,进行...

Study on Residue Elimination of Enrofloxacin Suspension in Pigs

The residue depletion of enrofloxacin(ENR) and ciprofloxacin(CIP) in the pig tissues was studied by using HPLC method.Enrofloxacin suspension was given in consecutive three days.After the last feeding,five pigs were slaughtered for collecting tissue samples.Tissue samples were extracted by phosphate buffer solution,purified through C18 SPE.Then the extract was dissolved with a mobile phase: 0.05 mol/L phosphoric acid/triet hylamine-acetonitrile(82+18).Finally,the samples were detected by HPLC after filtration.Results showed that,when the sample concentrations were 20 μg/kg to 2 000 μg/kg,the linear relationship was good.The detection limit was 20 μg/kg.The recovery was between 70% to 90%,and the coefficient of variation was under 10%.The depletion of enrofloxacin in muscle and fat was fast,and the sum of concentration of ENR and CIP was declined,lower than LOD on the 3rd day.However,compared with fat and muscle,the depletion of drugs in liver and kidney were slower.The sum of concentration of these in kidney was 185.7 μg/kg at the 3rd day.In integrating total residues in tissues and MRL rule,it suggested that enrofloxacin suspension withdrawal time in pig is 5 days.