苎麻CCoAOMT基因全长cDNA克隆与序列分析

【目的】试图分离和克隆苎麻内源咖啡酰辅酶A甲基转移酶基因。【方法】采用RACE技术克隆基因，对序列应用ClustaW 1.82软件进行在线分析，将苎麻mRNA序列、mRNA编码区序列以及氨基酸序列与已报道的其它植物的相应序列进行多重序列排列比较并构建系统树。【结果】获得了苎麻CCoAOMT cDNA全长序列(GenBank注册号：AY651026)；苎麻咖啡酰辅酶A甲基转移酶是氧甲基转移酶类；苎麻CCoAOMT基因mRNA序列与已报道的其他植物的相应序列不能聚为一类，其差异明显大于其它植物间的差异。苎麻CCoAOMT基因mRNA编码区序列与玉米（Zea mays：ZMA242980、ZMA242981）的同源性高于其他植物。推导的苎麻CCoAOMT酶蛋白氨基酸序列与杨树（Populus balsamifera：AJ224894、AJ224896）、美洲山杨（Populus tremuloides：PTU27116）的同源性高于其他植物。【结论】苎麻CCoAOMT基因cDNA与其它植物的相应序列具有同源性。

Cloning Full Length of Ramie Caffeoyl-CoA 3-O-Methyltransferase cDNA and Sequence Analysis

【Objective】 The full length of ramie(Boehmeria nivea) caffeoyl-CoA 3-O-methyltransferase cDNA was cloned. 【Method】 RACE (Rapid Amplification of cDNA Ends) method was used to analyze the sequence by ClustaW 1.82 software on line.【Result】 The full length of ramie caffeoyl-CoA 3-O-methyltransferase cDNA was cloned for the first time (GenBank accession number: AY 651026). The result of domain analyze showed that it is a member of O-methyltransferases family. Compared ramie mRNA sequence, mRNA CDS sequence and putative amino acid sequence CCoAOMT to other higher plants, phylogenetic tree of CCoAOMT was generated by multiple alignment. The result displayed that mRNA level of ramie can not be clustered into any other plant, but the result showed that ramie and Zea mays (ZMA242980, ZMA242981) have higher homology in mRNA CDS than other higher plants, while the amino acid sequence of ramie and populus (AJ224894, AJ224896, PTU27116) has higher homology than other plants.【Conclusion】Ramie (Boehmeria nivea) caffeoyl-CoA 3-O-methyltransferase cDNA has homology with that of other plants.