番茄青枯病抗性两个相关基因功能的初步鉴定

对抗性番茄材料‘Hawaii 7996’分别接种强致病力青枯菌（RsH）和中等致病力青枯菌（RsM）后，利用双向电泳技术找到了两个差异表达蛋白质--谷胱甘肽S转移酶L3和Remorin 1蛋白。在利用携带八氢番茄红素脱氢酶基因PDS的TRV重组病毒载体通过叶片浸润法证明VIGS体系可行后，进一步利用该技术沉默上述两个蛋白质的对应基因GST-L3和Rem-1，并用半定量RT-PCR检测沉默效果。结果表明，分别沉默GST-L3和Rem-1的番茄植株抗性减弱，在接种中等致病力青枯菌（RsM）后青枯病发病率和病情指数都明显高于对照植株，说明基因GST-L3和Rem-1与番茄对青枯病的抗性相关。

Studies of Two Genes Related to Bacterial Wilt Resistance in Tomato

Two-dimensional electrophoresis（2-DE）was employed to analyze the comparative proteome from stems of resistant tomato‘Hawaii 7996’plants inoculated with medium virulent strain RsM and high virulent strain RsH. Two differentially expressed proteins were identified to be Glutathione S-transferase L3 and Remorin 1 and the function of corresponding genes，GST-L3 and Rem-1，were further studied. After confirming the effectiveness of VIGS system with TRV carrying the Phytoene Desaturase gene（PDS），two targeted genes were individually silenced in Hawaii 7996 using VIGS method and their expression levels were checked by semi-quantitative RT-PCR. The results showed that the average disease incidence and disease index of bacterial wilt on GST-L3 or Rem-1 silenced plants increased significantly when challenging with RsM strain，indicating that these two genesare involved in the resistance of tomato to bacterial wilt disease.