松潘乌头嫩茎高效再生体系的建立

以松潘乌头（Aconitum sungpanense）嫩茎切段为外植体进行不定芽诱导和植株再生试验。松潘乌头初生嫩茎长至5 ~ 10 cm时将其切下作为外植体诱导不定芽，MS + 6-BA 4.0 mg ? L-1 + NAA 1.0 mg ? L-1是较适合的分化培养基，分化率达94.8 %，平均芽数4.2；不定芽增殖以MS + 6-BA 3.0 mg ? L-1 + IBA 0.3 mg ? L-1效果较好，在此增殖培养基中加入青霉素G钾5 mg ? L-1，GA3 0.3 mg ? L-1，蔗糖为50 g ? L-1，琼脂7 g ? L-1，对玻璃化苗的控制效果较好，增殖倍数可达7.26，苗高达4 cm左右；生根培养基以1/2MS + IAA 0.3 mg ? L-1较为理想，平均生根数为10条左右，生根率为96%以上；瓶苗移栽于森林土和草碳（1∶1）混合基质中生长良好，成活率达90%以上。

The Establishment of the Efficient Regeneration System of the Tender Stems of Aconitum sungpanense

The tender stems of Aconitum sungpanense were used as the explants to make a study of the adventitious bud induction and the plantlet regeneration. The results are as follows：The tender stems with the length of 5–10 cm are the ideal material for the explant culture. When the adventitious buds are inducted by the tender stems，the medium of MS + 6-BA 4.0 mg ? L-1 + NAA 1.0 mg ? L-1 is beneficial for the differentiation and the differentiation rate reaches 94.8%，besides，the number of the average bud is 4.2. The medium of MS + 6-BA 3.0 mg ? L-1 + IBA 0.3 mg ? L-1 is suitable for the propagation of buds. Meanwhile，to make the control of the vitrification of seedlings，it is effective to add Benzylpenicillin Potassium with 5 mg ? L-1 and GA3 0.3 mg ? L-1 to the original propagation medium and it is ideal to adopt sucrose with 50 g ? L-1 and agar with 7 g ? L-1 so that buds can be multiplied by 7.26 times and the height of seedlings can rise to about 4 cm as well. The optimum medium for rooting is 1/2MS + IAA 0.3 mg ? L-1 and the average number of rooting is approximately 10，besides，the rooting rate increases to over 96%. The seedlings transplanted to the matrix with forest soil and grass carbon（the ratio of 1︰1）grow vigorously and the survival rate reaches over 90%.