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茄子果实性状相关基因的QTL定位
引用本文:葛海燕,刘 扬,陈火英. 茄子果实性状相关基因的QTL定位[J]. 园艺学报, 2015, 42(11): 2197-2205. DOI: 10.16420/j.issn.0513-353x.2015-0492
作者姓名:葛海燕  刘 扬  陈火英
作者单位:上海交通大学农业与生物学院,上海 200240
基金项目:国家自然科学基金青年科学基金项目(31301770);上海市科技兴农种业项目[沪农科种字(2013)第5 号]
摘    要:以果实性状差异显著的茄子(Solanum melongena L.)种质材料绿茄‘131'和上海本地长茄‘141'为作图亲本,构建了237个株系的F_2群体,采用450对SSR标记构建遗传图谱,定位茄子果实单果质量、果长、果径、果形指数和果实花萼大小的QTL。研究结果表明:在亲本间表现多态性的SSR标记为63对,平均多态性比例为14%;共有53个标记被分配到13个连锁群上(12条染色体),13个连锁群覆盖总长度为532.2cM,标记间距范围为0.2~45.9 cM,平均距离为10.04 cM;共定位了9个与果实性状相关的QTL位点(单果质量1个,果长1个,果径2个,果形指数3个,果实花萼大小2个)。

关 键 词:茄子  遗传图谱  SSR  果实性状

QTL Analysis of Fruit-Associated Traits in Eggplant
GE Hai-yan,LIU Yang,CHEN Huo-ying. QTL Analysis of Fruit-Associated Traits in Eggplant[J]. Acta Horticulturae Sinica, 2015, 42(11): 2197-2205. DOI: 10.16420/j.issn.0513-353x.2015-0492
Authors:GE Hai-yan  LIU Yang  CHEN Huo-ying
Affiliation:School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China
Abstract:Two morphologically and molecularly distinguished advanced breeding lines,Lü Qie(Solanum melongena L.)‘131’and Shanghai Qie(Solanum melongena L.)‘141’,were selected formapping parents. An F2 population including 237 individuals was obtained. A total of 450 pairs of SSRmarkers were used to construct linkage map and therefore to locate QTL related to fruit weight,fruitlength,fruit diameter,fruit shape index and fruit calyx size. The results showed that only 63(14%)markersshowed polymorphism between two parents. A total of 53 SSR markers were mapped into 13 linkagegroups,including 12 chromsomes. The map spanned 532.2 cM. The distance between loci varied from 0.2to 45.9 cM,with the average distance of 10.04 cM. Nine QTLs for fruit-related traits were detected,including 1 QTL for fruit weight,1 QTL for fruit length,2 QTLs for fruit diameter,3 QTLs for fruit shapeindex and 2 QTLs for fruit calyx size.
Keywords:eggplant  linkage map  SSR  fruit trait
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