与甘蓝中心柱长相关联的EST-SSR标记分析

利用40 个EST-SSR 标记，对100 份甘蓝自交系组成的自然群体进行遗传结构分析，采用TASSEL3.0 软件的GLM（general linear model）和MLM（mixed linear model）模型，对甘蓝中心柱长和中心柱长/球高比值性状进行关联分析。结果显示供试材料可分为3 个亚群；EST-SSR 位点间有较高的多态性和一定程度的连锁不平衡；以GLM 模型分析，共检测出5 个标记的7 个位点与中心柱长性状相关联，2 个标记的3 个位点与中心柱长/球高性状相关联，其中2 个位点同时与两个性状相关联；以MLM 模型分析，共检测出3 个标记的4 个位点与中心柱长性状相关联，1 个标记的2 个位点与中心柱长/球高性状相关联，其中2 个位点同时与两个性状相关联。GLM 和MLM 两种模型同时检测到了与中心柱长/球高相关联的1 个标记的2 个位点以及与中心柱长相关联的3 个标记的4 个位点。

Analysis on EST-SSR Markers Associated with Core Length in Cabbage

Forty EST-SSR markers were applied for the genetic structure analysis of a natural population comprised 100 cabbage inbred lines，and association analysis between markers and two agronomic traits on core length were subsequently performed using GLM（general linear model）and MLM（mixed linear model）programs of TASSEL3.0 software in this study. Population genetic structure analysis showed that 100 cabbage accessions were composed of 3 subpopulations，and high polymorphism and various degree of linkage disequilibrium were detected among EST-SSR loci. There were 7 alleles of 5 markers associated with core length，and 3 alleles of 2 markers associated with core length/vertical diameter of head，and 2 alleles associated simultaneously with two observed traits under GLM program. There were 4 alleles of 3 markers associated with core length，and 2 alleles of 1 marker associated with core length/vertical diameter of head，and 2 alleles associated simultaneously with two observed traits under MLM program. Furthermore，2 alleles of 1 marker associated with core length/vertical diameter ofhead and 4 alleles of 3 markers associated with core length were determined under GLM and MLM programs.