葡萄中盐诱导的R2R3-MYB 基因的筛选与表达分析

 以葡萄砧木‘1103P’为试材，筛选获得了4 个受盐强烈诱导的R2R3-MYB 基因，系统进化 分析表明其编码蛋白属于不同的拟南芥R2R3-MYB 亚组。该4 个基因在根、茎、叶和果实中呈组成型表 达，但具有不同的表达水平，尤其是VvMYB112 在葡萄根系中具有较高的表达水平。在4 个基因中， VvMYB112 能响应100 ~ 200 mmol · L-1 高盐处理。此外，VvMYB112 和VvMYB15 也能被聚乙二醇（PEG6000） 和低温诱导，而VvMYB107 和VvMYB87 对PEG6000 和低温不敏感或受其抑制。表明这4 个基因可能通 过不同的途径介导抗盐性。

Screening and Expression Analysis of Salt-induced R2R3-MYB Genes in Grapevine

In the study，we obtained four R2R3-MYB genes which were strongly induced by salt in the roots of grapevine rootstock‘1103P’. The proteins encoded by the above four genes were clustered into the different subgroups in the phylogenetic tree of Arabidopsis R2R3-MYB proteins. The four genes were expressed in root，stem，leaf and berry，whereas they showed the different expression levels in the different tissues. For example，VvMYB112 was expressed predominantly in the root. Among the four genes，only VvMYB112 could be induced by 100–200 mmol · L-1 salt. Besides，VvMYB112 and VvMYB15 were induced by PEG and cold as well，while VvMYB107 and VvMYB87 did not respond to or were repressed by PEG6000 or cold. Taken together，the four screened genes showed the different expression patterns in the different tissues or under the different stress treatment，which indicated that they might mediate the salt resistance by the different pathways.