茄子SmNAC1 基因的克隆与表达分析

 以茄子幼苗为试材，利用cDNA 末端快速扩增（RACE）技术获得SmNAC1 基因全长，并利用RT-qPCR 技术检测该基因在GA、4 ℃低温、NaCl 处理下的时空表达。结果显示：SmNAC1 全长序列1 279 bp，开放阅读框为909 bp，编码302 个氨基酸，推测其蛋白质分子量约35.04 kD；SmNAC1 含有NAC 家族的N 端保守域，与番茄、马铃薯同源性高达90%。SmNAC1 受GA、低温和高盐诱导，表达上调，但在根、茎、叶中的表达量有差异，在根中表达水平整体高于茎和叶中，显示SmNAC1 在根中优势表达，且短时间（0.5 ~ 1 h）相对表达量较高，而在茎和叶中应答相对迟缓，且叶中表达水平趋于稳定。可见SmNAC1 可能参与了茄子对外源GA 诱导的响应及对低温、高盐胁迫的耐受调节过程。

Cloning and Expression Analysis of SmNAC1 in Solanum melongena

Eggplant seedlings were used as experimental materials and the full-length of SmNAC1was cloned using Rapid-amplification of cDNA ends（RACE）. The results showed that the full-lengthsequence of SmNAC1 is 1 279 bp and the open reading frame is 909 bp which encoding a polypeptide of302 amino acids with a protein molecular weight of about 35.04 kD. SmNAC1 contains the N-terminalconserved domain of NAC family and homology was as high as 90% with tomato and potato. Theexpression patterns were analyzed by Real-time quantitative PCR（RT-qPCR）under GA，4 ℃ and NaCltreatment. The results showed that SmNAC1 was induced by GA and stress such as low temperature andhigh salt treatment. However，there were some differences of SmNAC1 gene expression in roots，stems andleaves. The overall level of expression in the roots was higher than stems and leaves under differenttreatments，which indicated that SmNAC1 was predominantly expressed in root. The relative expressionlevel of SmNAC1 reached a high level with short time（0.5–1 h），while the reaction was relatively slow instems and leaves. The relative expression level had the smallest variations in leaves under different treattimes. The results suggested SmNAC1 may participate response of GA-induced and adjustment of lowtemperature and high salt stress tolerance in eggplant.