仁用杏品种SRAP遗传多样性分析及指纹检索系统的开发

利用SRAP标记对24份仁用杏品种进行了遗传多样性分析。15对引物共扩增出280条带，其中241条为多态性谱带，多态性比率为85.34%；每个引物组合扩增谱带数在13 ~ 24条之间，平均为18.7条；多态性信息含量（PIC）在0.28 ~ 0.65之间，平均为0.51。基于引物Me4-Em4扩增的多态性谱带，构建的指纹检索系统可以区分24个仁用杏品种。根据SRAP扩增结果，利用UPGMA法构建树状聚类图，在相似系数为0.70处可将24个仁用杏品种分为4组，聚类结果与形态分类基本一致。

Analysis of Genetic Diversity and Development of Fingerprinting Key Among Accessions of Kernel-using Apricot Using SRAP Markers

In order to evaluate the genetic relationship among accessions of kernel-using apricot，sequence related amplified polymorphism（SRAP）markers were used to evaluate genetic diversity of 24 samples. Fifteen primer pairs generated 280 bands，241 bands（85.34%）of which were polymorphic ones. SRAP primer pairs amplified 13 to 24 bands with an average of 18.7 bands per primer pair. Polymorphic information content（PIC）ranged from 0.28 to 0.65 with an average of 0.51. A fingerprinting key，established on the base of polymorphic bands amplified by a primer pair of Me4-Em4，could discriminate 24 accessions of kernel-using apricot. A dendrogram was constructed based on SRAP data using UPGMA culster method. The 24 accessions were classified into 4 major groups at the genetic similarity coefficient of 0.70，which was basically corresponded with the genetic relationship based on morphological traits.