辣椒全基因组WRKY转录因子的分析

基于已公布的辣椒全基因组数据,利用生物信息学方法对辣椒WRKY转录因子家族进行全面鉴定和系统命名,并在此基础上对基因分类、染色体定位、系统进化关系和结构域序列保守性进行了研究。结果表明:辣椒CaWRKY家族包含71个基因,根据WRKY结构域的数量及锌指结构的特征可将其分为GroupⅠ、GroupⅡ和GroupⅢ等3大类,GroupⅡ又可分为Ⅱ(a)、Ⅱ(b)、Ⅱ(c)、Ⅱ(d)和Ⅱ(e)等5个亚类。辣椒12条染色体上均有WRKY转录因子分布,其中第1号染色体上分布最多,共有10个,第4号染色体上分布最少,仅有2个。辣椒每类/亚类WRKY几乎含有相同的保守基序。辣椒WRKY编码的蛋白在132~869个氨基酸范围内,平均氨基酸数量为373个。

Genome-wide Analysis of the WRKY Transcription Factor Family in#br#Pepper

In the present study，based on the recently released pepper whole-genome sequences，CaWRKY gene family identification，gene classification，chromosome location，sequence alignment andconserved structure domains of CaWRKY proteins were predicted and analyzed with bioinformaticsmethods. The results showed that 71 CaWRKY genes were identified which were classified into three maingroups（Ⅰ，Ⅱand Ⅲ），with the second group further divided five subgroups[Ⅱ（a），Ⅱ（b），Ⅱ（c），Ⅱ（d）and Ⅱ（e）]. A total of 70 CaWRKY genes were mapped to 12 chromosomes，whereas onlyCaWRKY70 was not mapped to any particular chromosome. Genome mapping analysis revealed thatpepper WRKY genes were enriched on several chromosomes（1，2，3 and 7），especially on chromosome 1which encompasses the largest number of 10 CaWRKY genes，while chromosome 4 only contained 2CaWRKY genes. The pepper WRKYs from each group or subgroup were shown to share similar motifcompositions，and CaWRKY proteins contained 132–869 amino acids and 373 in average. Our results willprovide a platform for functional identification and molecular breeding tudy of WRKY genes in pepper.