| 茶多酚生产水相中茶氨酸分离技术研究 |
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| 引用本文： | 张星海,周晓红,陆旋,王岳飞. 茶多酚生产水相中茶氨酸分离技术研究[J]. 茶叶科学, 2008, 28(6) |
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| 作者姓名： | 张星海 周晓红 陆旋 王岳飞 |
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| 作者单位： | 浙江经贸职业技术学院应用工程系,浙江,杭州,310018;浙江大学茶学系,浙江,杭州,310029 |
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| 基金项目： | 浙江省供销社2007年科学研究项目 |
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| 摘 要： | 研究一种从生产茶多酚后的残留液中提纯茶氨酸的制备工艺;首先通过ZTC-II型天然澄清剂对茶多酚生产废液絮凝处理,离心液经过三种规格中空超滤膜超滤,透过液用一种特制的弱极性大孔树脂(JAD-2000)初步分离,制备含量在60%以上的茶氨酸粗品,再通过C18制备柱对其溶液进行分离纯化,制备含量在98%以上高纯度茶氨酸。结果表明经过絮凝和膜超滤处理,蛋白质和果胶等杂物去除率分别达到97%、89%以上,而茶多酚、可溶糖去除率不到9%,茶氨酸保留率达到93%;JAD-2000初步分离可得含量在61%以上粗茶氨酸,回收率达到71%以上,而醇洗馏份中茶氨酸含量甚微。经C18柱制备色谱分离粗茶氨酸样液,收集12.6~15.8min高浓度段馏份,检测知茶氨酸的回收率为71.3%,茶氨酸含量达98.3%。
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| 关 键 词： | 茶氨酸 生产废液 ZTC 超滤 大孔树脂 |
Study on the Isolation Technology of Theanine from Aqueous Phase of Tea Polypheonls Production |
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| ZHANG Xing-hai,ZHOU Xiao-hong,LU Xuan,WANG Yue-fei. Study on the Isolation Technology of Theanine from Aqueous Phase of Tea Polypheonls Production[J]. Journal of Tea Science, 2008, 28(6) |
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| Authors: | ZHANG Xing-hai ZHOU Xiao-hong LU Xuan WANG Yue-fei |
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| Affiliation: | ZHANG Xing-hai1,ZHOU Xiao-hong1,LU Xuan1,WANG Yue-fei2 (1. The Department of Applied Engineering,Zhejiang Economic , Trade Polytechnic,Hangzhou 310018,China,2. Department of Tea Science,Zhejiang University,Hangzhou 310029,China) |
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| Abstract: | The preparative technology of theanine from the aqueous phase of tea polypheonls production was investigated. Firstly, retreating the aqueous liquids with ZTC-II, then, Separating the clarifying liquids by three kinds of ultra-filtration, the crude theanine (>60%) can be obtained from the filtrated liquid by using the JAD-2000 which is a kind of macroporous resin with weak polarity. High purity theanine (>98%) can be obtained through preparative high performance liquid chromatography (prep-HPLC) with C18 co... |
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| Keywords: | ZTC |
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