NDGA对红颜草莓脱落酸及其关键调控基因表达的影响

采用不同浓度的ABA合成抑制剂NDGA处理红颜草莓果实,通过测定草莓果实不同时期脱落酸及成熟着色相关生理指标以及ABA代谢相关基因的变化,以了解NDGA对脱落酸及其关键调控基因表达的影响。结果表明,NDGA能有效抑制可溶性糖和天竺葵素葡萄糖苷的合成,且在一定范围内浓度越高,抑制作用越明显。一定浓度的NDGA处理可以抑制FaNCED的表达,并促进FaCYP707A、FaBG1和FaCHLH在草莓果实褪绿期的表达。该结果为进一步揭示NDGA抑制脱落酸代谢的机制提供了研究基础。

Effect of NDGA treatment on ABA content and expression of related genes in strawberry

The Benihoppe strawberry was treated with different concentrations of nordihydroguaiaretic acid (NDGA), which can depress the synthesis of abscisic acid (ABA) to investigate its influence on physiological indexes and related gene expression. The results showed that NDGA could significantly inhibit biosynthesis of soluble sugar and pelargonidin 3-O-glucoside chloride, and the inhibition became more obvious with the increasing concentration. In addition, FaNCED was inhibited and FaCYP707A, FaBG1 and FaCHLH were accelerated with a certain concentration of NDGA. Our study may provide helpful information for further study on molecular mechanism of NDGA on inhibiting the biosynthesis of ABA.