金银花尺蠖蛹粗多糖体外抗氧化活性

为了解金银花尺蠖Heterolocha jinyinhuaphaga蛹粗多糖的抗氧化活性，采用热水浸提法提取了金银花尺蠖蛹粗多糖，并测定其体外抗氧化活性。结果表明:金银花尺蠖蛹粗多糖对二苯代苦味酰自由基（DPPH），羟基自由基（·OH）以及超氧阴离子自由基（O₂-·）具有一定的清除作用，在其质量浓度为0.2 g·L-1时的清除率分别为39.32%，35.62%和26.75%。随着多糖质量浓度的增大，对自由基的清除率也逐渐增加，当质量浓度上升到1.2 g·L-1时，清除率分别为74.93%，61.59%和47.84%，分别增加了35.61%，25.97%和21.09%，差异达显著水平（P < 0.05）。在0.2~1.2 g·L-1的质量浓度范围内，清除率与粗多糖质量浓度之间呈显著的线性关系。金银花尺蠖蛹粗多糖具有较强的体外抗氧化活性，具有开发为抗氧化剂的潜力。图 4 参20

Antioxidant activity of raw polysaccharides from Heterolocha jinyinhuaphaga pupae in vitro

To understand the antioxidant activity of raw polysaccharides from the Heterolocha jinyinhuaphaga pupae, and provide scientific reference for development of natural antioxidant drugs and performance foods, raw polysaccharides from the pupae was extracted in the laboratory by the hot water extraction method. Then, its antioxidant activity was studied in vitro by spectrophotometric method. Its clearing rate on 2, 2-diphenyL-1-picrylhydrazyl (DPPH), ·OH and O₂-. was tested with 3 replications, and vitamin C was used as control. Results showed that with a dosage of 0.2 g·L-1, the clearing rate of raw polysaccharide was 39.3% on DPPH, 35.6% on ·OH, and 26.8% on O₂-. With a 1.2 g·L-1 dosage, the clearing rate increased to 74.9% for DPPH, 61.6% for ·OH, and 47.8% for O₂-. From 0.2 to 1.2 g·L-1, the clearing rate showed a linear connection to the dosages. Thus, the pupae polysaccharide of H. jinyinhuaphaga. had a high antioxidant activity, which could potentially be exploited.［Ch, 4 fig. 20 ref.］