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鲍蒸煮液美拉德反应条件的优化
引用本文:吴靖娜,路海霞,靳艳芬,位绍红,刘智禹.鲍蒸煮液美拉德反应条件的优化[J].浙江农业学报,2016,28(1):157.
作者姓名:吴靖娜  路海霞  靳艳芬  位绍红  刘智禹
作者单位:(1. 福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,福建 厦门 361013; 2. 福建省海洋生物资源开发利用协同创新中心,福建 厦门 361013; 3. 集美大学 食品与生物工程学院,福建 厦门 361021)
摘    要:以鲍蒸煮液为原料,利用美拉德反应获得具有特殊风味的产物。在单因素实验的基础上,利用响应面法对美拉德反应工艺进行优化,建立了温度、时间、糖浓度与褐变程度、中间产物、游离氨基酸含量之间的数学模型。根据模拟得到的二次回归模型确定了美拉德反应的最佳工艺条件:反应温度115 ℃、反应时间84 min、糖(葡萄糖∶木糖=2∶3)浓度5%,得到的反应液为红褐色、烧烤味浓郁,此时D420=0.552、D294=0.653、游离氨基酸含量3.93 mg·mL-1。

关 键 词:鲍蒸煮液  美拉德反应  响应面法  

Optimization of Maillard reaction conditions of abalone cooking liquor
WU Jing\|na,LU Hai\|xia,JIN Yan\|fen,WEI Shao\|hong,LIU Zhi\|Yu.Optimization of Maillard reaction conditions of abalone cooking liquor[J].Acta Agriculturae Zhejiangensis,2016,28(1):157.
Authors:WU Jing\|na  LU Hai\|xia  JIN Yan\|fen  WEI Shao\|hong  LIU Zhi\|Yu
Institution:(1. Fisheries Reasearch Instiute of Fujian, Key Laboratory of Cultivation and High\|value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; 2. Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361013, China; 3. College of Food and Biological Engineering, Jimei University, Xiamen 361021, China)
Abstract:Abalone cooking liquor, Maillard reaction product with special flavor, was obtained through Maillard reaction. On the basis of single factor experiments, response surface method was used to optimize Maillard reaction process, and the mathematical model among temperature, time, sugar concentration and the degree of browning, intermediate, free amino acid content, was established. The technological conditions were optimized by the quadratic regression model, which were temperature 115 ℃, time 84 min, sugar concentration (glucose∶xylose=2∶3) 5%. In such conditions, the reaction liquid was red brown with strong toasty, and the indexes of Maillard reaction were measured as D420=0.552, D294=0.653, and free amino acid content was 3.93 mg·mL-1.
Keywords:abalone cooking liquor  Maillard reaction  response surface method  
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