丙肝感染宿主亲嗜性基因OCLN和CD81在猕猴川西亚种组织中的分布

丙型肝炎动物模型缺乏限制了丙型肝炎的研究，如果与人类基因背景相似的猕猴能作为丙型肝炎动物模型将有重大意义。文章拟通过检测决定HCV感染物种特异性的关键基因OCLN与CD81在猕猴不同组织的表达量，并以HCV易感的人肝癌细胞系Huh 751为对照，来探究猕猴作为丙型肝炎动物模型的可能性。结果表明，OCLN与CD81在猕猴川西亚种的肝脏、脾脏、肺脏、淋巴结和脊髓中均有表达，但表达量均极显著低于在Huh 751细胞系中的表达量（P<0.01）。肝脏中的OCLN表达量略低于肺脏中的表达量，而肝脏CD81的表达量均高于其他组织。并且，OCLN在不同组织中的表达水平与人体OCLN表达规律相符合（脾＜肝＜肺）。该结果提示HCV感染的宿主亲嗜性基因OCLN与CD81在猕猴中的低表达量可能导致HCV不能有效地结合组织靶细胞，因此推测，猕猴不能直接用于丙型肝炎动物造模可能与此有关。今后可通过转基因等技术增强OCLN与CD81基因的表达，从而使猕猴获得直接感染HCV的能力。

Distribution of HCV infection related OCLN and CD81 in different tissues of Macaca mulatta lasiotis

The study on hepatitis C virus （HCV） was restricted by lack of suitable animal model. Macaca mulatta lasiotis， known as macaque which has the similar genetic background with human， may be a suitable animal model for studying HCV infection. By testing the expression level of species tropism gene OCLN and CD81 in different macaque tissues， the possibility of using macaque as an effective animal model was explored with the expression level of HCV\|susceptible human liver cancer cell line of Huh 751 as the control. The results showed that OCLN and CD81 were expressed in liver， spleen， lungs， lymph nodes and spinal cord of macaque， but the expression levels were significantly lower than that in Huh 751（P＜0.01）. The expression of OCLN in liver was lower than that in lung， but CD81 expression in liver was higher than other tissues. In addition， the expression of OCLN in different tissues of macaque showed a similar pattern as that in human， with a decreasing order of lung＞liver＞spleen. It was concluded that the low expression level of OCLN and CD81 in macaque could lead to the ineffective combination of HCV and target tissue， which was possibly related to the inefficiency of macaque as a suitable animal model for HCV. In future， the expression level of OCLN and CD81 in macaque may be enhanced by genetic manipulation， so as to improve the HCV\|infection of macaque.