光肩星天牛对寄主的选择及主要寄主挥发物的化学成分分析

为了探寻寄主植物气体挥发物对光肩星天牛Anoplophora glabripennis寄主选择的影响机制,分别对健康的木麻黄Casuarina equisetifolia,白蜡Fraxinus chinensis,垂柳Salix babylonica,苦楝Melia azedarach,意大利杨树Populus euramevicana ‘I-214’枝条挥发物进行成分分析。室内"Y"型嗅觉实验结果显示:5种树种对天牛引诱程度不同。与对照相比,5种树种挥发物对光肩星天牛的引诱效果差异显著(P＜0.05),苦楝和垂柳对光肩星天牛的引诱效果差异极显著(P＜0.01)。气相色谱-质谱联用技术(GC-MS)分析鉴定结果显示:柳树、杨树、苦楝气体挥发物中共同含有乙酸丁酯、壬醛、莰烯、α-蒎烯,木麻黄与白蜡中发现2,4-二叔丁基苯酚、1,3-二乙基苯乙酮及樟脑。推测光肩星天牛对5种树种的选择差异可能与萜烯类化合物种类,烷烃、醇类、酮类的相对含量有关。

Anoplophora glabripennis host-plant selection with main host-plant volatile chemical component analysis

To explore host-plant influence on the Asian long-horned beetle (Anoplophora glabripennis), five different healthy host branches, including Casuarina equisetifolia, Fraxinus chinensis, Salix babylonica, Populus euramevicana ‘I-214’,and Melia azedarach, were used for a composition analysis with an indoor "Y"-tube olfactometer experiment. Host-plant volatiles from the host plants were identified by gas chromatography-mass spectrometry (GC-MS). Comparing with control group, results reflected that the attraction of the five host plants to A. glabripennis were significantly different (P＜0.05) with M. azedarach and S. babylonica highly significant (P＜0.01). GC-MS results showed differences in chemical fingerprinting. A. glabripennis had the strongest attraction to S. babylonica, M. azedarach, and P. euramevicana ‘I-214’ and were attracted to butyl acetate, nonyl aldehyde, camphene, and alpha pinene that existed in the three host plants. Camphor and 2,4-2 tertiary butyl phenol, a repellent to A. glabripennis, were identified in F. chinensis. A great amount of 1, 3-diethyl acetophenone was found in C. equisetifolia, explaining its poor attraction for A. glabripennis offerring more theoretical basis for study and preparation of attractant and repellent to A. glabripennis.