草莓MYB类转录因子FaMYB5基因的克隆与表达分析

以丰香草莓（Fragaria ×ananassa cv. Toyonaka）为试材，通过同源克隆技术获得FaMYB5基因全长编码序列，运用生物学软件对该序列进行相关生物信息学分析，同时采用实时荧光定量PCR方法对FaMYB5基因在果实不同发育阶段的表达模式进行研究。FaMYB5基因的cDNA全长为822 bp，编码273个氨基酸，蛋白质分子量为29159 ku，等电点为5709，与森林草莓的FvMYB5同源性达957%。实时荧光定量PCR分析显示，随着果实发育成熟，FaMYB5基因的表达逐渐上升。在果实的小绿期（SG），FaMYB5基因的表达量最低。但是在白果期（WT）出现1个降低的峰值，其表达量略高于小绿期，草莓果实开始转红时其表达量又逐渐上升，在果实全红期（FR）达到最大。结果获得了丰香草莓MYB类转录因子FaMYB5基因的cDNA全长，其表达存在差异。

Cloning and expression analysis of MYB transcription factor FaMYB5 gene from strawberry

The full length cDNA sequence of FaMYB5 gene was cloned from strawberry（Fragaria×ananassa cv. Toyonaka）through homology cloning method. Then， bioinformatics and expression pattern of FaMYB5 were analyzed by some softwares and real time fluorescent quantitative PCR technique， respectively. Sequence analysis showed that the cDNA of FaMYB5 was 822 bp， encoding 273 amino acids， while the estimated molecular weight and isoelectric point of the putative protein were 29159 ku and 5709， and shared high identity of 957% with FvMYB5 from woodland strawberry. The expression analysis showed that the expression level of FaMYB5 was gradually increasing during fruit development. The lowest expression was detected at small green (SG) stage, and a reduced peak appeared at white stage, when the expression level was merely higher than SG stage. Thereafter, the expression increased gradually from initially red(IR) stage to the full red(FR) stage when the highest level was detected. In short, full length cDNA of FaMYB5 was cloned from strawberry, and the expression levels were different during fruit development.