桑天牛成虫血淋巴凝结抑制剂的筛选

选用柠檬酸钠等8种药剂进行了对桑天牛Apriona germari成虫血淋巴凝结的抑制试验。冷藏血淋巴溶解时按V（血淋巴）:V（抑制剂）:V（苯基硫脲）=1:1:1和1:2:2的用量加入抑制剂，4 ℃下静置2 min和6 h，各药剂抑制血淋巴凝结结果为:按V（血淋巴）:V（抑制剂）:V（苯基硫脲）=1:2:2的用量加入质量分数为10 g·kg-1柠檬酸钠，凝结抑制效果最好，柠檬酸-磷酸二氢钠溶液、草酸钾、秋水仙碱、任氏液、磷酸缓冲液也有一定的抑制作用，而噻孢霉素和硫酸新霉素的抑制作用很小。按1:2:2的用量加入10 g·kg-1柠檬酸钠，可更有效抑制冷藏血淋巴解冻时的凝结。新鲜血淋巴采集时按V（血淋巴）:V（抑制剂）:V（苯基硫脲）=1:2:2的用量加入抑制剂，6 h后观察表明:10 g·kg-1柠檬酸钠的凝结抑制效果最好；任氏液、磷酸缓冲液、柠檬酸-磷酸氢钠缓冲液处理的血淋巴，有少量沉淀；其余4种药剂处理的血淋巴均有大量沉淀和凝块产生。用柠檬酸钠处理桑天牛成虫血淋巴，可有效抑制其血淋巴的凝结。表 7 参14

Hemolymph coagulation inhibitor screening for use on Apriona germari

Adding suitable agglutination inhibitor has helpful for long-term preservation of hemolymph and for physiological and biochemical research of hemolymph. Hemolymph agglutination inhibition tests on adult Apriona germari were conducted using sodium citrate and seven other drugs. During dissolving of cold storage hemolymph, mixtures (by volume) of hemolymph, inhibitor and phenylthiourea were made and then placed at 4 ℃. Inhibition effects of every inhibitor mixture (citric acid-natrium, dihydrogen phosphate buffer, potassium oxalate, colchicine, Ringer solution, and a phosphate-buffered saline (PBS) buffer), were determined after being applied for 2 min and for 6 h. Through the naked eye observation and microscopic examination, the shape and quantity of sediment and the blood cells were intact to evaluate the effect of anticoagulation. Results showed that the inhibition effect of 1% sodium citrate in the mixture of hemolymph, sodium citrate, and phenylthiourea (1:2:2 by volume) was best. Using citric acid-natrium, dihydrogen phosphate buffer, potassium oxalate, colchicine, Ringer solution, and a phosphate-buffered saline (PBS) buffer as inhibitors, hemagglutination in these mixtures was also inhibited. Inhibition of Cefotaxime and Sulfate Neomycin were very small. After collection and being placed for 6 h, of all inhibitors the inhibition effect of 1% sodium citrate was best. A small amount of precipitate was found in mixtures of Ringer solution, PBS buffer, citric acid-natrium, and dihydrogen phosphate when used as inhibitors. Much precipitate and hemolymph coagulation were found in the other four inhibitors' mixtures. Therefore, by using sodium citrate as a hemolymph inhibitor for adult Apriona germari, hemagglutination could be effectively inhibited.[Ch, 7 tab. 14 ref.]