SgP5CS基因克隆和生物信息学分析

利用同源克隆的方法，获得了碱蓬P5CS基因全长ORF，命名为SgP5CS。SgP5CS ORF全长2 151 bp，生物信息学软件预测其编码716个氨基酸组成的多肽，相对分子量为77 431.8 u， 等电点为5.71，为稳定的亲水性蛋白，无跨膜结构域。ClustalX多重序列比对发现SgP5CS编码的氨基酸序列与盐角草P5CS相似性为93%，与盐角草P5CS的亲缘关系最近，其次是甜菜。脯氨酸是生物体内重要的渗透调节剂，而Δ1 吡咯啉 5 羧酸合成酶则是植物合成脯氨酸的关键酶之一，SgP5CS的克隆将为进一步的功能分析鉴定基础。

Molecular cloning and bioinformatics analysis of SgP5CS gene in Suaeda glauca

A new gene， named as SgP5CS， was cloned by homologous cloning method from Suaeda glauca. Bioinformatics analysis showed that the open reading frame of SgP5CS gene was 2 151 bp， encoding 716 amino acids. The isoelectric point and the relative molecular weight of coded protein were 5.71 and 77.4318 u， respectively. Hydrophobic analysis indicated that SgP5CS was a hydrophilic protein and had no transmembrane domain. Nucleotide sequence Blast by ClustalX indicated that the coded protein of SgP5CS shared an identity of 93% with P5CS of Salicornia europaea. Δ1 pyrroline 5 carboxylate synthetase （P5CS） is a key enzyme in the synthesis of proline which is one of the important osmotic regulators in plants. Our research laid a foundation to further study the P5CS function in Suaeda glauca.