中国荷斯坦牛LF基因多态性与泌乳性状及乳腺炎的关联分析

乳铁蛋白（Lactoferrin,LF）是保护乳腺组织的防御因子之一，在机体抵抗乳腺炎方面起着重要作用。本研究采用PCR SSCP技术和测序的方法对宁夏农垦303头中国荷斯坦牛的LF基因进行了遗传多态性分析，利用一般线性模型分析LF基因g.4388G>C位点突变对日产奶量、测定日乳脂率、测定日乳蛋白率及体细胞评分4个泌乳性状的影响。结果显示，LF基因g.4388G>C突变位点与日产奶量、乳脂率和体细胞评分值存在显著关联（P<0.05）。多重比较结果表明，GC基因型个体的体细胞评分（SCS）值极显著低于GG和CC基因型个体（P<0.01），因此,可尝试将GC基因型作为乳腺炎抗性的优良基因型应用于奶牛育种中。

Genetic polymorphism of LF gene and its associations with milk traits and mastitis of Chinese Holstein cattle

Lactoferrin（LF） is one of the defense factor in breast tissue， which plays an important role in bodys resistance to mastitis. The polymorphism of LF gene in 303 Holstein cattle in Ningxia was detected by PCR SSCP， and the effects of LF gene g.4388G>C locus mutation on daily milk yield， milk fat， milk protein percentage and somatic cell score （SCS） were analyzed via general linear model. It was shown that there was a significant relationship between LF gene g.4388 G>C locus mutation and milk yield， milk fat and SCS （P<0.05）. The GC genotype had significantly lower SCS compared with GG and CC genotypes （P<0.01）. Therefore， GC gene as a superior genotype against mastitis would be applied in dairy cattle breeding.