Monitoring of the Roundup Ready soybean in the Vojvodina province in Serbia期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Monitoring of the Roundup Ready soybean in the Vojvodina province in Serbia

Authors:	Zorica Nikolić Ksenija Taški-Ajduković Mladen Tatić Svetlana Balešević-Tubić

Affiliation:	1. University of California, Berkeley, Department of Environmental Science, Policy & Management, Berkeley, CA 94720-3114, USA;2. ILPLA (CONICET-CCT La Plata) — UNLP Instituto de Limnología “Dr. Raúl A. Ringuelet”, Boulevard 120 y 62, La Plata, 1900, Buenos Aires, Argentina;3. Laboratório de Avaliação e Promoção da Saúde Ambiental, IOC, FIOCRUZ, Av. Brasil 4365 Manguinhos, Rio de Janeiro, RJ 21045-900, Brazil;4. Center for Fisheries, Aquaculture and Aquatic Sciences, Department of Zoology, Southern Illinois University, Carbondale, IL 62901, USA;1. Department of Plant Science, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea;2. Division of Conservation Ecology, Bureau of Ecological Conservation Research, National Institute of Ecology, Seocheon-gun, Choongnam 33657, Republic of Korea;3. Department of Agricultural Biotechnology, National Institute of Agricultural Academy, Rural Development Administration, Wanju-gun, Jeonbuk 55365, Republic of Korea;4. Department of Horticultural Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju-gun, Jeonbuk 55365, Republic of Korea

Abstract:	The Vojvodina province is the most important agricultural area of the Republic of Serbia and is its largest soybean producer. Serbian low forbids the introduction of genetically modified organisms (GMO) into the environment and demands labeling of food containing more than 0.9% GMO. This aim of this study is to monitor the Roundup Ready (RR) soybean in fields and in food products using PCR and immunoassays. A duplex PCR reaction was performed to analyze the lectin gene and 35S promoter using DNA isolated from soybean leaf and seed sample. Specific detection of RR markers was performed using nested PCR and verified by immunoassay. RR soybeans were found in 68 fields in samples from 2003 and 28 fields in 2004. The presence of the RR soybean was confirmed in 44 seed samples out of 7142 samples examined in 2006 and in 108 out of 7171 samples examined in 2007. The data presented here are the first to demonstrate the presence of RR soybean in the Serbia fields.

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