啶氧菌酯降解菌的分离鉴定及其降解特性

通过富集培养法筛选分离到1株能以啶氧菌酯为唯一碳源的降解菌株PID-1,采用形态学、生理生化方法,并结合16S rDNA序列系统发育分析,将菌株PID-1初步鉴定为沼泽红假单胞菌(Rhodopseudomonas palustris)。菌株PID-1降解啶氧菌酯的最佳条件为pH 7和35 ℃。在该降解条件下,培养5 d,菌株PID-1对100 mg·L^-1啶氧菌酯的降解率可达83.54%。将啶氧菌酯经PID-1降解后的物质经质谱扫描,通过谱库检索,发现其降解中间产物包括1-(1,5-dimethylhexyl-)-4-methyl-benzene、2,5-bis (1,1-dimethylethyl)-phenol、butyl 2-methyoxyethyl ester、bis (tert-butyldimethylsilyl) ester、1-(3-n-propoxyphenyl)-2-propanone oxime和2-nitro-4-(trifluoromethyl) phenol。

Isolation and degrading characteristics of picoxystrobin degrading strain

The strain PID-1 was screened and isolated by enrichment culture, and was primarily identified as Rhodopseudomonas palustris based on morphology, physiological and biochemical characteristics as well as phylogenetic analysis of 16S rDNA sequences. The optimal degrading conditions of PID-1 were pH 7 and 35 ℃. Under this condition, up to 83.54% of 100 mg·L^-1 picoxystrobin could be degraded by PID-1. The metabolics of picoxystrobin degraed by PID-1 were identified by mass spectrum as 1-(1,5-dimethylhexyl-)-4-methyl-benzene, 2,5-bis (1,1-dimethylethyl)-phenol, butyl 2-methyoxyethyl ester, bis (tert-butyldimethylsilyl) ester, 1-(3-n-propoxyphenyl)-2-propanone oxime and 2-nitro-4-(trifluoromethyl) phenol.