不同杂交家系铁皮石斛花多糖、浸出物及氨基酸质量分数分析

铁皮石斛Dendrobium officinale花是铁皮石斛药材的非药用部位，为测定其多糖、浸出物、氨基酸的质量分数，探讨3种成分之间的内在关系，全面了解铁皮石斛花中的物质分布规律，利用药典方法测定铁皮石斛花的多糖、浸出物质量分数，并采用17种氨基酸标准品，通过酸水解法提取、异硫氰酸苯酯（PITC）柱前衍生处理样品，结合反相高效液相色谱法（RP-HPLC）测定10个不同杂交家系铁皮石斛花中的氨基酸组成与质量分数。结果表明:铁皮石斛花中多糖质量分数平均为71.00 mg·g-1，浸出物质量分数为300.00 mg·g-1；从铁皮石斛花中检测到16种氨基酸，总氨基酸平均质量分数为7.88%；3种成分质量分数差异显著（P < 0.05）。利用SPSS 17.0分析3种成分之间的相关性，多糖和浸出物在合成积累上呈现负相关，与氨基酸之间相关性不明显（P>0.05）。铁皮石斛花中含有较丰富的多糖、浸出物及氨基酸等营养及功效成分，具有一定的保健开发价值。图 1 表 3 参11

Polysaccharides,extracts, and amino acids in hybrid Dendrobium officinale flowers

Dendrobium officinale flowers, which have many non-medicinal folk applications, were studied to determine the distribution and correlation of substances. Using pharmacopeia methods 1) the polysaccharide content, 2) the extracts, and 3) the amino acid content were determined by acid hydrolysis and phenyl iso-thiocyanate (PITC) pre-column derivatization combined with a Reversed-phase-high-performance liquid chromatography (RP-HPLC) assay. SPSS 17.0 was used for the analysis. Results showed D. officinale flowers had an average of 71.00 mg·g-1 polysaccharides and 300 mg·g-1 extracts; 16 kinds of amino acids having 7.9% of total amino acids were found. The SPSS 17.0 analysis for the relationship of the three ingredients revealed a negative correlation for the synthetic accumulation between polysaccharide and extracts, but no significant correlation with amino acids. In all, D. officinale flowers had nutritional and functional components including polysaccharides, extracts, and amino acids offering potential health benefits.Ch, 1 fig. 3 tab. 11 ref.]