25味常用清热解毒药提取物对猪源幽门螺杆菌的体外抑菌活性

采用乙醇回流、超声波等方法提取金果榄等25味常用清热解毒中药的抗菌活性成分，分别测定它们对猪源幽门螺杆菌（Helicobacter pylori，Hp）的抑菌圈直径和最小抑菌浓度（minimal inhibitory concentration， MIC），筛选出对猪源Hp具有良好体外抑菌效果的几味中药，并评价其体外联合抑菌效果。结果显示，黄连和青果的提取物对猪源Hp的MIC范围为1.56～3.12 mg·mL-1。金银花、金果榄、大青叶、玄参、山豆根、白头翁、紫花地丁、大黄和蒲公英提取物的MIC范围为12.50～25.00 mg·mL-1。大血藤、虎杖、射干、忍冬藤、菊花、鱼腥草、连翘、板蓝根、益母草、诃子和白蔹提取物的MIC范围为50.00～100.00 mg·mL-1。升麻、绵马贯众、柴胡提取物的MIC≥200.00 mg·mL-1。两两联合抑菌试验结果表明，青果和金果榄的联合抑菌指数（FICI）≤0.5，青果或金果榄分别与黄连联合的0.5＜FICI≤1，黄连、青果、金果榄分别与金银花联合的FICI＞2。本研究表明，黄连、青果、金银花、金果榄对猪源Hp均有很强的体外抗菌活性，青果与金果榄为协同作用，青果或金果榄分别与黄连联合时为相加作用，黄连、青果、金果榄分别与金银花联合时均为拮抗作用。

Effects of twenty|five traditional antipyretic and antidote medicine extracts against Helicobacter pylori from swine in vitro

 Using ethanol reflux， water decoction or ultrasonic method， extracts of 25 different traditional antipyretic and antidote medicines were obtained. Inhibition zone diameters of above 25 extracts on Helicobacter pylori（Hp） from swine were detected by Oxford cup method. Minimal inhibitory concentration（MIC） were detected by macrodilution broth method， and combined antibacterial effects in vitro of them were also observed. MICs of the extracts obtained from rhizome coptidis， fructus canarii against Hp ranged from 156 to 312 mg·mL-1. MICs of the extracts obtained from flos lonicerae， radix tinosporae， folium isatidis， radix scrophulariae， radix sophorae tonkinensis， radix pulsatillea， herba violae， radix et rhizome rhei and herba taraxaci ranged from 12.50 to 25.00 mg·mL-1. MICs of the extracts obtained from caulis sargentodoxae， rhizome polygoni cuspidati， rhizoma belamcandae， caulis lonicerae， flos chrysanthemi， herba houttuyniae， fructus forsythiae， radix isatidis， herba leonuri， fructus chebulae and radix ampelopsis ranged from 50.00 to 100.00 mg·mL-1. MICs of the extracts obtained from rhizome cimicifugae， rhizome dryopteris crassirhizomatis and radix bupleuri exceeded 200.00 mg·mL-1. The results of pairwise joint bacteriostatic test showed that the fractional inhibitory concentration index （FICI） of the extracts from fructus canarii and radix tinosporea against Hp was less than or equal to 0.5. The FICI of fructus canarii/radix tinosporea with rhizome coptidis were more than 0.5， but less than or equal to 1. The FICI of fructus canarii/radix tinosporea/rhizome coptidis with flos lonicerae were more than 2. It was concluded that fructus canarii， radix tinosporea， rhizome coptidis， flos lonicerae showed a good antibacterial effect against Hp in vitro. The combination of fructus canarii and radix tinosporea was characterized by a synergism effect， whereas the combination of fructus canarii and rhizome coptidis， radix tinosporea and rhizome coptidis， respectively， was characterized by an additive effect. The combination of fructus canarii or radix tinosporea or rhizome coptidis with flos lonicerae showed a antagonism effect.