海藻糖浸种对盐胁迫下扬麦19生理特性的影响

以扬麦19为材料，研究4个海藻糖溶液浓度（0、2、20、40 mmol·L-1）浸种对其种子萌发的影响。选择对种子萌发没有明显抑制效应的最适海藻糖溶液浓度（2 mmol·L-1），研究其对盐胁迫下（3 g·L-1 NaCl）扬麦19幼苗生长及生理特性的影响。结果表明，2 mmol·L-1海藻糖溶液浸种处理对扬麦19种子发芽率和幼苗株高无显著影响，但对幼苗单株干质量有显著促进作用。在盐胁迫下，2 mmol·L-1海藻糖浸种处理可显著提高扬麦19幼苗相对生长率、单株生物量、叶绿素相对含量和N含量，比水浸种处理分别提高200%、650%、1507%和925%；幼苗脯氨酸积累速度明显加快，K+含量及K+/Na+显著增加，Na+含量显著下降（P<005）。双因素方差分析表明，影响幼苗K+含量的程度依次为海藻糖>NaCl>海藻糖×NaCl。海藻糖浸种处理可以使扬麦19更快更早地积累脯氨酸，增强对K+的吸收，以适应盐胁迫环境。

Effects of soaking seeds in trehalose on physiological characteristics of wheat Yangmai 19 under salt stress

To investigate the effect of trehalose pretreatment with different concentrations of 0， 2， 20 and 40 mmol·L-1 on seed germination， Yangmai 19， a high yielding winter wheat cultivar， was selected as the experimental material. Furthermore， the optimal concentration of trehalose （2 mmol·L-1） was screened out to study its effect on growth and physiological characteristics of Yangmai 19 under salt stress （3 g·L-1 NaCl）. The results showed that the seed germination and plant height were not significantly affected in the pretreatment with 2 mmol·L-1 trehalose， but the dry weight per plant was significantly increased. Under salt stress， compared with the non treated seeds， the relative growth rate， biomass per plant， relative chlorophyll content and N content in the pretreatment of 2 mmol·L-1 trehalose were significantly improved by 200%， 650%， 1507% and 925%， respectively. Proline accumulation rate was found to be significantly increased in the seedlings pretreated with 2 mmol·L-1 trehalose than the non treated seeds， as well as the total K+ content and K+/Na+ ratio， while Na+ content was significantly decreased （P<005）. Two factor variance analysis showed that the factor orders of impacting K+ content was trehalose>NaCl>trehalose×NaCl. Trehalose pretreatment could improve the seedlings adaption to salt stress earlier， by increasing their proline accumulation and also increasing their K+ absorption.