不同品种鸭胚胎期骨骼肌成肌细胞GHR和IGF 1 mRNA表达差异分析

为探讨生长激素受体（growth hormone receptor，GHR）和胰岛素样生长因子 1（Insulin like growth factor 1，IGF 1）在不同品种鸭胚胎期骨骼肌成肌细胞中的表达差异。选择生长速度不同的金定鸭和高邮鸭为试验动物，采用实时荧光定量PCR方法检测鸭13，15，17，19，21和23胚龄时胸肌和腿肌成肌细胞GHR，IGF 1 mRNA的表达情况，并进行了品种间比较。结果发现：13胚龄时，高邮鸭胸肌和腿肌成肌细胞IGF 1的表达均显著高于金定鸭（P<0.05）；17胚龄是两个品种鸭GHR和IGF 1共同的表达高峰期；17胚龄之后，鸭胸肌和腿肌成肌细胞GHR和IGF 1表达均显著降低。在19，21胚龄时，高邮鸭胸肌成肌细胞2个基因的表达均显著高于金定鸭（P<0.05），而腿肌成肌细胞GHR和IGF 1表达在品种间无显著差异（P>0.05）。鸭胚胸肌和腿肌成肌细胞GHR和IGF 1表达呈现极显著的正线性相关（P<0.01）。提示：GHR和IGF 1的表达谱及各自在品种间的变化规律基本一致；鸭胚骨骼肌成肌细胞GHR和IGF 1 mRNA表达有组织特异性，二者之间可能存在正调控的作用机制。

The expression analysis of GHR and IGF 1 mRNA in embryonic skeletal muscle myoblasts of different duck breeds

To investigate the expression patterns of growth hormone receptor （GHR） and insulin like growth factor 1 （IGF 1） in duck skeletal muscle myoblasts， fluorescent real time quantitative PCR was used to detect the distribution profile of GHR and IGF I mRNA in skeletal muscle myoblasts of Jinding and Gaoyou ducks at the embryonic day 13， 15， 17， 19， 21 and 23. The results showed that the expressions of IGF 1 of Gaoyou ducks in breast and leg muscle myoblasts were significantly higher than that of Jinding ducks at embryonic day 13 （P<0.05）; the embryonic day 17 was the common expression peak of GHR and IGF 1 both in Gaoyou ducks and Jinding ducks; and then， significantly decreased. But at the embryonic day 19 and 21， the expressions of GHR and IGF 1 in Gaoyou ducks breast muscle myoblasts were significantly higher than those of Jinding ducks （P<0.05）， while there was no significant difference between varieties in the leg muscle myoblasts （P>0.05）. The expression of GHR had a significantly linear positive correlation with the expression of IGF 1 in breast and leg muscle myoblasts（P< 0.01）. The results showed that the expression pattern of GHR was consistent with the expression of IGF 1 between breeds; the expressions of GHR and IGF 1 mRNA in the skeletal muscle myoblasts of duck embryo showed tissue specificity， and there might be a positive regulation mechanism between them.