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| 锌指核酸酶技术在动物转基因研究中的应用 | |
| 引用本文： | 刘晓,方永志,刘文浩,王洪梅,何洪彬.锌指核酸酶技术在动物转基因研究中的应用[J].山东农业科学,2013,45(2):135-138. |
| 作者姓名： | 刘晓 方永志 刘文浩 王洪梅 何洪彬 |
| 作者单位： | 1. 山东师范大学,山东济南250014;山东省农业科学院奶牛研究中心,山东济南250131 2. 济南军区军犬训练大队,山东济南,250100 3. 山东省农业科学院奶牛研究中心,山东济南,250131 |
| 基金项目： | 泰山学者海外特聘专家(何洪彬),国家奶牛产业技术体系建设专项经费(何洪彬);山东省科技攻关课题(2009GG20002032);国家转基因重大专项(2009ZX08007-006B,2011ZX08007-002,2011ZX08008-004);济南市高校院所自主创新计划(201004027) |
| 摘 要： | 锌指蛋白核酸酶(Zinc Finger Nuclease,ZFN)是一种人工合成酶,含有锌指蛋白和FokⅠ核酸内切酶的剪切结构域,因其能特异性识别并切割DNA序列及其可设计性而被用基因定点突变和外源基因定点整合。这项技术已应用于转基因动物研究中。本文综述了锌指核酸酶技术在转基因研究中的应用进展,并对锌指核酸酶技术的应用前景进行了展望。 |
| 关 键 词： | 锌指蛋白 锌指核酸酶 基因打靶 同源重组 转基因动物 |
Application of Zinc Finger Nuclease in Animal Transgenic Research |
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| Liu Xiao , Fang YongZhi , Liu WenHao , Wang HongMei , He HongBin.Application of Zinc Finger Nuclease in Animal Transgenic Research[J].Shandong Agricultural Sciences,2013,45(2):135-138. | |
| Authors: | Liu Xiao Fang YongZhi Liu WenHao Wang HongMei He HongBin |
| Institution: | 1.College of Life Science,Shandong Normal University,Jinan 250014,China; 2.Cow Research Center,Shandong Academy of Agricultural Sciences,Jinan 250131,China; 3.Army Dog Training Group,Jinan Army Military,Jinan 250100,China) |
| Abstract: | Zinc finger nuclease(ZFN) is a kind of artificial synthesis containing zinc finger protein and the shear structure domain of FokⅠ endonuclease.Because it can specifically identify and cleave DNA sequences and easily be designed,it is used for the site-directed genetic mutations and integration of exogenous genes.This technology has been applied to the research of transgenic animals.In this paper,the research progress of zinc finger nuclease technology and its application were reviewed,and its application prospect was also forecasted. |
| Keywords: | Zinc finger protein Zinc finger nuclease Gene targeting Homologous recombination Transgenic animal |
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