Embryo development and fruit-set in pear induced by untreated and irradiated pollen |
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Authors: | Renata Sniezko T. Visser |
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Affiliation: | (1) Institute of Biology, University Marie Curie-Sklodovska, Lublin, Poland;(2) Institute for Horticultural Plant Breeding (IVT), Wageningen, the Netherlands |
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Abstract: | Summary Flowers of the pear cultivars Conference, Doyenné du Comice and Gieser Wildeman were pollinated with untreated and 50-krad-irradiated pollen. Fruitlets were sectioned at regular intervals up to 6 weeks after pollination. Tubes of the irradiated pollen grew slower, but within two weeks nearly as many embryos were initiated as in the case of untreated pollen. Thereafter, the proportion of degenerated embryos increased, although the endosperm sometimes persisted. Six weeks after pollination only about 21% of the seeds induced by the irradiated pollen still contained normal embryos. At or before that time in-vitro culture of the immature seeds will be necessary because few embryos survive until harvest. The irradiated pollen stimulated a rather large parthenocarpic fruit-set, presumably associated with endosperm rather than embryo development during the first 4–6 weeks after pollination. The possibilities of haploid production are discussed. |
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Keywords: | Pyrus pear irradiated pollen embryogenesis fruit-set haploids |
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