| 普洱茶抗氧化特性的初步研究 |
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| 引用本文： | 揭国良,何普明,丁仁凤. 普洱茶抗氧化特性的初步研究[J]. 茶叶, 2005, 31(3): 162-165 |
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| 作者姓名： | 揭国良 何普明 丁仁凤 |
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| 作者单位： | 浙江大学茶叶研究所,杭州,310029;浙江大学茶叶研究所,杭州,310029;浙江大学茶叶研究所,杭州,310029 |
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| 摘 要： | 本研究用不同的有机溶剂，分步萃取普洱茶的水提物，得到氯仿萃取物、乙酸乙酯萃取物、正丁醇萃取物和萃取后剩余物。采用Fe^2-H2O2-鲁米诺的化学发光法和有机自由基二苯基苦基偕腙肼（DPPH）的分光光度法，比较各提取物的清除自由基的能力。结果 提示普洱茶水提物中的乙酸乙酯萃取层组分和正丁醇萃取层组分对DPPH和羟自由基均有较强的清除能力。
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| 关 键 词： | 普洱茶 抗氧化 化学发光法 |
| 文章编号： | 0577-8921(2005)03-162-04 |
| 收稿时间： | 2005-04-04 |
| 修稿时间： | 2005-06-05 |
Abecedarian study on the antioxidant activity of pu-er tea |
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| JIE Guoliang,HE Puming,DING Renfeng. Abecedarian study on the antioxidant activity of pu-er tea[J]. Journal of Tea, 2005, 31(3): 162-165 |
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| Authors: | JIE Guoliang HE Puming DING Renfeng |
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| Abstract: | The pu-er tea extract treated with hot w at er was re-extracted with different organic regents step by step, got four kind of fractions, such as chloroform fraction, ethyl acetate fraction, n-butano l fraction and the other fraction. To compare the antioxidative activity of thes e fractions, the antioxidative effect was assayed in a Fenton-type reaction sys tem by chemiluminescence, and in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) rad ical scavenging effect by spectrophotometer. The result showed that the strong s cavenging effect of the ethyl acetate fraction and the n-butanol fraction o n the DPPH radical and the hydroxyl radicals was observed. |
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| Keywords: | Pu-er tea Antioxidant activity Chemiluminescence assay |
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