| 番茄POD与EST同工酶PAGE方法研究 |
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| 引用本文： | 陈启林,巩振辉,陈静. 番茄POD与EST同工酶PAGE方法研究[J]. 西北农业学报, 1999, 8(1): 87-90 |
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| 作者姓名： | 陈启林 巩振辉 陈静 |
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| 作者单位： | 西北农业大学园艺系, 陕西杨陵 712100;西北农业大学园艺系, 陕西杨陵 712100;西北农业大学园艺系, 陕西杨陵 712100 |
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| 摘 要： | 对番茄种子芽期过氧化物酶（ＰＯＤ）同工酶与酯酶（ＥＳＴ）同工酶聚丙烯酰胺凝胶电泳（ＰＡＧＥ）方法进行了初步研究。结果表明，ＰＯＤ同工酶电泳酶样提取液以ｐＨ６．５￣０．１ｍｏｌ／Ｌ磷酸缓冲液＋０．１％巯基乙醇较适宜；ＥＳＴ同工酶酶样提取液以ｐＨ６．０￣７．０的０．１ｍｏｌ／ＬＴｒｉｓ－柠檬酸＋０．１％巯基乙醇或０．１ｍｏｌ／Ｌ磷酸缓冲液＋０．１％巯基乙醇较适宜。常用的７．５％分离胶浓度不适宜番茄种子
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| 关 键 词： | 番茄 过氧化物酶 酯酶 同工酶 电泳 品种 |
| 收稿时间： | 1997-10-13 |
| 修稿时间： | 1997-12-22 |
Study on PAGE Method of POD and EST Isozyme in Tomato Seed |
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| Chen Qilin,Gong Zhenhui and Chen Jing. Study on PAGE Method of POD and EST Isozyme in Tomato Seed[J]. Acta Agriculturae Boreali-occidentalis Sinica, 1999, 8(1): 87-90 |
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| Authors: | Chen Qilin Gong Zhenhui Chen Jing |
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| Affiliation: | Northwestern Agricultural University, Yangling Shaanxi 712100;Northwestern Agricultural University, Yangling Shaanxi 712100;Northwestern Agricultural University, Yangling Shaanxi 712100 |
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| Abstract: | Study on PAGE method of POD and EST isozyme in tomato germinating seeds was carried out,the results indicated that the suitable extraction buffer for POD isozyme is pH6.5~7.5 0.1 mol/L, phosphate buffer +0.1% mercaptoethanol,and that for EST isozyme is pH 6.0~7.0 0.1 mol/L. Triscitrate +0.1% mcrcaptoethanol or 0.1 mol/L phosphate buffer +0.1 mol/L. mercaptoethanol. The 7.5% separating gel concentration was not suitable for both isozymes in question,it should be replaced with 11.5% concentration in order to achieve satisfactory electrophoresis effect. |
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| Keywords: | Tomato POD EST Isozyme electrophoresis |
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