| 山桃原生质体培养再生愈伤组织 |
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| 引用本文： | 马锋旺,李嘉瑞.山桃原生质体培养再生愈伤组织[J].西北农业学报,1999,8(3):73-76. |
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| 作者姓名： | 马锋旺 李嘉瑞 |
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| 作者单位： | 西北农业大学园艺系, 陕西杨陵712100;西北农业大学园艺系, 陕西杨陵712100 |
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| 摘 要： | 以山桃县浮培养物为分离材料，在ＣＰＷ＋１．０％ＥＬＬＵＬＡＳｏＮＺＵＫＡｒ－１０＋０．５％ＭａｃｅｒｏｚｙｍｅＲ－１０＋０．７ｍｏｌ／Ｌ甘露醇＋１％ＰＶＰ的酶解液中酶解１２ｈ生质体产量和活力分别达到３．５＊１０＾７个／ｇ．ＦＷ和９６％。
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| 关 键 词： | 山桃 原生质体分离 原生质体培养 |
| 收稿时间： | 1998/4/23 0:00:00 |
| 修稿时间： | 1998/7/10 0:00:00 |
Callus Formation from Cultured Protoplasts of Prunus Davidiana |
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| MA Feng-wang and LI Jia-rui.Callus Formation from Cultured Protoplasts of Prunus Davidiana[J].Acta Agriculturae Boreali-occidentalis Sinica,1999,8(3):73-76. |
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| Authors: | MA Feng-wang and LI Jia-rui |
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| Institution: | Department of Horticulture, Northwestern Agricultural University, Yangling Shaanxi 712100;Department of Horticulture, Northwestern Agricultural University, Yangling Shaanxi 712100 |
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| Abstract: | The protoplast yield and viability of Prunus davidiana suspension cultures could amount to 3.5×107 protoplasts/g×FW and 96% respectively if incubated in enzyme solution containing CPW salts,1.0% Cellulase Onzuka R-10,0.5% Macerozyme R-10,0.7 mol/L mannitol and 1% polyvinylpirrolidone for 12 hours.KM8P medium with 1.0 mg/L 2,4-D and 0.1 mg/L BA was a suitable culture medium.The osmotic pressure should be reduced progressively.Microcalli were developed after 60 days culture in a liquid layer with plating density of 3×105/ml.The microcalli grown rapidly when subcultured on solid medium,but no adventitious bud was developed on diffentiation medium. |
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| Keywords: | Prunus davidiana Protoplast isolation Protoplast culture |
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