| 通过根癌农杆菌介导法获得新疆甜瓜抗病优质新品系 |
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| 引用本文： | 李仁敬,孙严,许健,伊鸿平,孟庆玉,翟文强,冯炯鑫,危晓薇,吴明珠,喻梅辉,陈章良. 通过根癌农杆菌介导法获得新疆甜瓜抗病优质新品系[J]. 西北农业学报, 1999, 8(1): 3-6 |
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| 作者姓名： | 李仁敬 孙严 许健 伊鸿平 孟庆玉 翟文强 冯炯鑫 危晓薇 吴明珠 喻梅辉 陈章良 |
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| 作者单位： | [1]新疆农科院核技术生物技术研究所农业部西北农作物细胞 [2]新疆农科院园艺作物研究所 |
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| 摘 要： | 用新疆甜瓜无菌苗子叶块,通过根癌农杆菌介导法,将黄瓜花叶病毒外壳蛋白ｃＤＮＡ基因导入“新红心脆”等６个品种（系）,经卡那霉素筛选,获得大量Ｋｍ抗性植株,其中部分Ｋｍ抗性植株经Ｌｕｉｓ法检测,表达了胭脂碱合成酶活性。进一步经ＳＤＳ－ＰＡＧＥ,ＤＯＴ－Ｅｌｉｓａ,ＰＣＲ特异扩增。Ｗｅｓｔｅｒｎ－Ｂｌｏｔ等方法检测,结果证明了Ｋｍ抗性植株基因组中整合了ＣＭＶ－ＣＰ基因,长度为１ｋｂ,并能有效表达,表达产
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| 关 键 词： | 新疆 甜瓜 根癌 农杆菌导法 抗病 优质新品系 |
| 收稿时间： | 1998-09-23 |
| 修稿时间： | 1998-11-13 |
A High Quality and Disease-resistant New Line of Xinjiang Melon Obtained by Agrobacterium Tumefacien-mediated Transformation |
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| Li Renjing,Sun Yan,Xu Jian,Yi Hongping,Meng Qingyu,Zhai Wenqiang,Feng Jiongxin,Wei Xiaowei,Wu Mingzhu,Yu Melhui and Pan Naisui. A High Quality and Disease-resistant New Line of Xinjiang Melon Obtained by Agrobacterium Tumefacien-mediated Transformation[J]. Acta Agriculturae Boreali-occidentalis Sinica, 1999, 8(1): 3-6 |
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| Authors: | Li Renjing Sun Yan Xu Jian Yi Hongping Meng Qingyu Zhai Wenqiang Feng Jiongxin Wei Xiaowei Wu Mingzhu Yu Melhui Pan Naisui |
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| Affiliation: | Institute of Nuclear &. Biological Technologies. XAAS, Key Laboratory of North-West Crop Cell Engineering, Ministry of Agriculture. Urumqi 830000;Institute of Nuclear &. Biological Technologies. XAAS, Key Laboratory of North-West Crop Cell Engineering, Ministry of Agriculture. Urumqi 830000;Department of Biological Sciences, Xinjiang Agricultural University;Institute of Horticulture Crop, Xinjiang Academy of Agriculture Sciences;Institute of Nuclear &. Biological Technologies. XAAS, Key Laboratory of North-West Crop Cell Engineering, Ministry of Agriculture. Urumqi 830000;Institute of Horticulture Crop, Xinjiang Academy of Agriculture Sciences;Institute of Horticulture Crop, Xinjiang Academy of Agriculture Sciences;Institute of Nuclear &. Biological Technologies. XAAS, Key Laboratory of North-West Crop Cell Engineering, Ministry of Agriculture. Urumqi 830000;Institute of Horticulture Crop, Xinjiang Academy of Agriculture Sciences;Department of Biological Sciences, Xinjiang Agricultural University;The National Laboratory of Protein Engineering and Plant Genetic Engineering Beijing University |
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| Abstract: | The cotyloden strips of six Xinjiang melon varieties(lines) "Xin Hongxincui" etc. were transformed with Cucumber Mosaic Virus Coat Protein (CMV-CP)gene by A. tumefacien-mediated method,and large amount of anti-kanamycin(kan)plant were obtainted. After test by Luis method, some anti-kan plants showed the activity of nopalin synthase. Further tests,such as:PCR,dot blot, western blot,etc.,indicated that the CMV-CP gene had integrated in the genomic DNA of the anti-kan plants,and the expression of this 1 kb gene in plant produced a protein of 24 kDa, an expected size for CMV-CP. The expression amount was about 5% of total plant protein. After traditional breeding of 9 generations, the new melon line K3-19 of high quality and disease-resistance of stable heridity was obtained from these anti-kan plants. |
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| Keywords: | Xinjiang melon Cucumber Mosaic Virus coat protein gene Transgenic plant Agrobacterium tumefacien-mdiated transformation New melon line of high quality and disease resistance |
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