| 无菌接种室洁净度测定简易技术 |
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| 引用本文： | 何金柱,郎凤红,杨贵荷等.无菌接种室洁净度测定简易技术[J].中国园艺文摘,2014(3):10-12. |
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| 作者姓名： | 何金柱 郎凤红 杨贵荷等 |
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| 作者单位： | 宁夏农垦葡萄苗木工程技术研究中心;宁夏农垦西夏王实业公司玉泉营葡萄基地分公司; |
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| 基金项目： | 致谢：本文在甘肃农业大学曹孜义教授指导下完成,并得到宁夏农垦西夏王实业有限公司高级工程师郭蕙萍的审阅和修改,在此一并致谢. |
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| 摘 要： | 总结在宁夏玉泉营进行酿酒葡萄茎尖培养、脱毒和无菌快繁建立无菌接种室和超净工作台洁净度的测定方法,按要求进行降落菌数测定计数和必要的措施等,能获得符合无菌接种室洁净度,并对洁净度的标准进行讨论,为沙区进行脱毒和快繁提供参考。
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| 关 键 词： | 酿酒葡萄 葡萄脱毒和快繁 洁净度 无菌操作 |
A simple technique for the determination of aseptic inoculation room cleanliness |
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| Institution: | HE Jin-zhu, LANG Feng-hong, YANG Gui-he, REN Yan-qing |
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| Abstract: | This paper reports a method for the determination of super clean sterile chamber and worktable for the wine grape stem tip culture, detoxification and rapid propagation in Ningxia Yuquanying. According to the requirements of determining the number of bacteria count and necessary measures, the cleanliness of asepsis inoculating room can be obtained. The cleanliness standards are discussion too. This study will offer a reference for detoxification and rapid propagation in desert area. |
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| Keywords: | wine grape grape detoxification and rapid propagation cleanliness aseptic operation |
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