Preparation and evaluation of antigens used in serological tests for caprine syncytial retrovirus antibody in sheep and goat sera |
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Authors: | W Coackley V W Smith D J Houwers |
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Affiliation: | Animal Health Laboratory, Department of Agriculture, South Perth, Western Australia 6151 Australia;Central Veterinary Institute, Department of Virology, Edelhertweg 15, 8129 PH Lelystad The Netherlands |
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Abstract: | Methods used to prepare antigens from caprine syncytial retrovirus (CSR) for use in the agarose gel immunodiffusion test (AGID) or an indirect enzyme-linked immunosorbent assay (ELISA) are described. Caprine and ovine sera were tested for antibody to CSR using the AGID test and ELISA incorporating a caprine system (CSR antigen and rabbit anti-goat IgG) or an ovine system (maedi-visna virus antigen and rabbit anti-sheep IgG). Good correlation was achieved in the results of the 3 tests when sera were devoid of antibody or were strongly positive. Variations in the results on weakly positive sera were considered to be more a matter of interpretation than due to basic differences in the reagents employed. |
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