| 板栗蛋白质的提取及多肽的制备 |
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| 引用本文： | 张帅,张培宜,冯翠萍. 板栗蛋白质的提取及多肽的制备[J]. 山西农业大学学报(自然科学版), 2011, 31(1): 73-76 |
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| 作者姓名： | 张帅 张培宜 冯翠萍 |
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| 作者单位： | 山西农业大学食品科学与工程学院,山西太谷,030801 |
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| 基金项目： | 山西省普通高等学校大学生创新性实验项目基金 |
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| 摘 要： | 以板栗为原料,提取蛋白质并水解制备多肽。通过SDS-PAGE测定板栗蛋白质的分子量,以植物蛋白水解专用酶采用三因素三水平的正交试验筛选制备板栗多肽的最佳水解条件,旨在为板栗的开发,利用提供理论依据。板栗蛋白质的等电点为4.5;分子量约为25 000 Da;植物蛋白水解专用酶最佳的水解条件:底物浓度2.5 g.L-1,酶添加量0.3%,pH 8.5,水解度是20.13%。得到板栗蛋白并制备出板栗多肽。
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| 关 键 词： | 板栗 蛋白质 多肽 植物蛋白水解专用酶 |
Extraction of Chinese Chestnut Protein and Preparation of the Ploypeptides |
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| ZHANG Shuai,ZHANG Pei-yi,FENG Cui-ping. Extraction of Chinese Chestnut Protein and Preparation of the Ploypeptides[J]. Journal of Shanxi Agricultural University(Nature Science Edition), 2011, 31(1): 73-76 |
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| Authors: | ZHANG Shuai ZHANG Pei-yi FENG Cui-ping |
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| Affiliation: | ZHANG Shuai,ZHANG Pei-yi,FENG Cui-ping(College of Food Science & Technology,Shanxi Agricultural University,Taigu Shanxi 030801,China) |
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| Abstract: | Protein was extracted from Chinese chestnut,and the Chinese chestnut polypeptide was obtained by hydrolysis,with a substance to provide a scientific basis for the development and use of Chinese chestnut protein polypeptide.Molecular weight of the protein was determined through SDS-PAGE.The albumen hydrolysis special-purpose enzyme was selected to determine the optimum hydrolysis conditions about Chinese chestnut polypeptide,using three factors three levels orthogonal experimental design.The isoelectronic po... |
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| Keywords: | Chinese chestnut Protein Polypeptides Special-purpose enzyme for plant albumen hydrolysis |
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