| 鲤鱼骨酶解工艺的优化 |
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| 引用本文： | 朱迎春,郭丽,王琪钰.鲤鱼骨酶解工艺的优化[J].山西农业大学学报(自然科学版),2011,31(2):179-183. |
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| 作者姓名： | 朱迎春 郭丽 王琪钰 |
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| 作者单位： | 山西农业大学食品科学与工程学院,山西太谷,030801 |
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| 基金项目： | 山西农业大学青年创新基金项目 |
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| 摘 要： | 为了充分利用骨资源,以鲤鱼头和鱼骨为原料,用无水乙醚、异丙醇、乙醇三种有机溶剂对其进行脱脂处理,以脱脂率为指标采用三因素三水平正交试验,确定最佳脱脂方法;利用胰蛋白酶、木瓜蛋白酶、中性蛋白酶对脱脂后的鱼骨粉进行水解,以水解度为测定指标,采用四因素三水平正交试验确定最佳酶解条件。结果表明,采用异丙醇在30℃条件下萃取120 min的脱脂效果最好;胰蛋白酶在底物浓度为1∶15,酶用量为5400 U.g-1,酶解4 h可获得最高水解度(19.20%)。
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| 关 键 词： | 鲤鱼骨 脱脂 酶解 |
Study on Enzymolysis Conditions of Carp Bone |
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| ZHU Ying-chun,GUO Li,WANG Qi-yu.Study on Enzymolysis Conditions of Carp Bone[J].Journal of Shanxi Agricultural University,2011,31(2):179-183. |
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| Authors: | ZHU Ying-chun GUO Li WANG Qi-yu |
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| Institution: | ZHU Ying-chun,GUO Li,WANG Qi-yu(College of Food and Science,Shanxi Agricultural Universiry,Taigu Shanxi 030801,China) |
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| Abstract: | In this experiment,we took the carp head and bone as materials and removed fat from them with three kinds of organic solvents including anhydrousether,isopropylalcohol and alcohol after pre-treatment.Based on the degreasing rate index,we designed orthogonal test in order to choose the best technology of defatting;Carp bone was hydrolyzed by three proteases(trypsin,papain and neutrase) and we chose the degree of hydrolysis as index to define the best condition of enzymolysis.The results showed that isopropyl... |
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| Keywords: | Carp bone Degreasing Enzymatic hydrolysis |
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