新干特早柚GPAT基因5'端克隆及序列分析

采用5'-RACE技术从新干特早柚(Citrus grandis cv. Xingantezaoyou)叶片中克隆出一条长832 bp的GPAT基因5'端cDNA片段(GenBank 登录号:DQ193970).序列分析结果表明:该cDNA片段序列与其它科属植物的GPAT基因序列的同源性差异较大,除与同类植物--温州蜜柑(Citrus unshiu)的同源性高达98.9%,与红花(Carthamus tinctorius)、拟南芥(Arabidopsis thaliana)、非洲油棕榈(Elaeis guineensis)、豌豆(Pisum sativum)、蚕豆(Vicia faba)的同源性分别为62.5%、54.6%、48.2%、47.4%、46.9%;其所编码的氨基酸序列与红花、拟南芥、非洲油棕榈、豌豆、辣椒的同源性分别为51.6%、60%、45.1%、48%、52.3%,与细菌(Bacillus amyloliquefaciens)的同源性仅为18.9%,说明GPAT基因的氨基酸序列具有种属特异性.

Cloning and Sequencing Analysis of the 5' Fragment of GPAT Gene from Citrus grandis cv. Xingantezaoyou

Citrus maxima cv. Xingantezaoyou' s GPAT gene of 5' end fragment was isolated from the leaf by means of 5 ' - RACE technology. The cDNA fragment is 832 bp long ( GenBank Accession No: DQ193970). The nucleotide sequence analysis showed that these sequences of GPAT 5' end fragment are greatly different from those of other plants. The best nucleotide similarity to Citrus unshiu was 98.9% but those Carthamus tinctorius, Arabidopsis thaliana ,Elaeis guineensis, Pisum sativum and Viciafaba were 62.5% ,54.6% , 48.2% ,47.4% and 46.9% respectively. The deduce amino acids sequence analysis showed the similarity to Carthamus tinctorius,Arabidopsis thaliana, Elaeis guineensis,Pisum sativum and Capsicum annuum were 51.6% , 60% ,45.1% ,48% and 52.3%. The amino acid sequences of GPAT gene has genera specificity because the similarity of amino acid sequences to Bacillus amyloliquefaciens is only 18.9%.