首页 | 本学科首页   官方微博 | 高级检索  
     检索      

3种鲟血清蛋白的比较研究
引用本文:张颖,孙大江,刘红柏.3种鲟血清蛋白的比较研究[J].大连水产学院学报,2006,21(3):283-286.
作者姓名:张颖  孙大江  刘红柏
作者单位:中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070;中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070;中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070
摘    要:采用聚丙烯酰胺凝胶电泳及饱和硫酸铵分步沉淀法对健康的3龄中华鲟Acipenser sinensisG ray、2龄施氏鲟A.schrenckiiB randt和3龄达氏鳇Huso dauricusGeorgi的血清蛋白及其组成成分进行了研究。结果表明,3种鲟的血清电泳图谱具有各自的特有谱带,其电泳图谱的相似程度表明,中华鲟与施氏鲟的亲缘关系较达氏鳇与施氏鲟近。3种鲟血清蛋白的总浓度分别为18.70、21.25、24.60 mg/mL。

关 键 词:  血清蛋白  电泳  浓度
文章编号:1000-9957(2006)03-0283-04
收稿时间:07 11 2005 12:00AM
修稿时间:2005-07-11

Electrophoretic analysis of serum proteins in three sturgeon (Acipenser sinesis Gruy, Huso dauricus Georgl,Acipenser schrencki Brandt)
ZHANG Ying,SUN Da-jing,LIU Hong-bai.Electrophoretic analysis of serum proteins in three sturgeon (Acipenser sinesis Gruy, Huso dauricus Georgl,Acipenser schrencki Brandt)[J].Journal of Dalian Fisheries University,2006,21(3):283-286.
Authors:ZHANG Ying  SUN Da-jing  LIU Hong-bai
Institution:Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070,China
Abstract:PAGE(polyacrylamide gel electrophoresis) and saturation ammonium sulfate sedimentation were used to analyze serum proteins of three sturgeons,2~ year old Amur sturgeon Acipenser schrenckii Brandt,3~ year old Chinese sturgeon Acipenser sinesis Gray and 3 years old Huso Beluga.Chinese sturgeon,Amur sturgeon,and Huso Beluga had total serum protein level of 18.70 mg/mL,21.25 mg/mL and 24.60 mg/mL,respectively.Relationship coefficient of serum protein showed that Amur sturgeon was more closer to Chinese sturgeon than to Huso Beluga.There were 8 bands in Chinese sturgeon,13 bands in Amur sturgeon and 8 bands in Huso Beluga by PAGE.
Keywords:sturgeon  serum protein  PAGE  protein level
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号